BMRB Entry 18691

Title:
Backbone 1H, 13C, and 15N backbone resonance assignments of the TPR1 domain of mouse STI1
Deposition date:
2012-08-30
Original release date:
2012-10-18
Authors:
Maciejewski, Andrzej; Choy, Wing-Yiu
Citation:

Citation: Maciejewski, Andrzej; Prado, Marco; Choy, Wing-Yiu. "1H, 15N, and 13C backbone resonance assignments of the TPR1 and TPR2A domains of mouse STI1."  Biomol. NMR Assignments 7, 305-310 (2013).
PubMed: 23070844

Assembly members:

Assembly members:
tpr1, polymer, 118 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: House mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pDEST17

Data sets:
Data typeCount
13C chemical shifts198
15N chemical shifts111
1H chemical shifts111

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1tpr11

Entities:

Entity 1, tpr1 118 residues - Formula weight is not available

1   GLYGLUGLNVALASNGLULEULYSGLULYS
2   GLYASNLYSALALEUSERALAGLYASNILE
3   ASPASPALALEUGLNCYSTYRSERGLUALA
4   ILELYSLEUASPPROGLNASNHISVALLEU
5   TYRSERASNARGSERALAALATYRALALYS
6   LYSGLYASPTYRGLNLYSALATYRGLUASP
7   GLYCYSLYSTHRVALASPLEULYSPROASP
8   TRPGLYLYSGLYTYRSERARGLYSALAALA
9   ALALEUGLUPHELEUASNARGPHEGLUGLU
10   ALALYSARGTHRTYRGLUGLUGLYLEULYS
11   HISGLUALAASNASNLEUGLNLEULYSGLU
12   GLYLEUGLNASNMETGLUALAARG

Samples:

sample_1: sodium phosphate 50 mM; sodium chloride 50 mM; DTT 1 mM; DSS 100 uM; tpr1, [U-13C; U-15N], 0.5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
REF XP_004713702

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks