BMRB Entry 18652

Title:
Backbone assignment of Bt-Lon alpha sub-domain from Brevibacillus thermoruber
Deposition date:
2012-08-09
Original release date:
2014-04-22
Authors:
Chen, Yu-Da; Hsu, Chun-Hua
Citation:

Citation: Lee, Alan Yueh-Luen; Chen, Yu-Da; Chang, Yu-Yung; Lin, Yu-Ching; Chang, Chi-Fon; Huang, Shing-Jong; Wu, Shih-Hsiung; Hsu, Chun-Hua. "Structural basis for DNA-mediated allosteric regulation facilitated by the AAA+ module of Lon protease."  Acta Crystallogr. D Biol. Crystallogr. 70, 218-230 (2014).
PubMed: 24531457

Assembly members:

Assembly members:
alpha_sub-domain, polymer, 124 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Brevibacillus thermoruber   Taxonomy ID: 33942   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Brevibacillus thermoruber

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-21a

Data sets:
Data typeCount
13C chemical shifts214
15N chemical shifts101
1H chemical shifts205

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1alpha sub-domain1

Entities:

Entity 1, alpha sub-domain 124 residues - Formula weight is not available

N-terminal M (490), and C-termianl LEHHHHHH (606-613) are artifacts sequencing and tag.

1   METALAGLYTYRTHRGLULEUGLULYSLEU
2   HISILEMETARGASPTYRLEULEUPROLYS
3   GLNMETGLUGLUHISGLYLEUGLYARGASP
4   LYSLEUGLNMETASNGLUGLUALAMETLEU
5   LYSVALILEARGGLNTYRTHRARGGLUALA
6   GLYVALARGASNLEUASNARGGLUALAALA
7   ASNILECYSARGLYSALAALAARGLEUILE
8   VALSERGLYGLULYSLYSARGVALVALVAL
9   THRPROLYSTHRVALGLUSERLEULEUGLY
10   LYSPROARGTYRARGTYRGLYLEUALAGLU
11   ARGGLUASPGLNVALGLYALAVALTHRGLY
12   LEUALATRPTHRGLNALALEUGLUHISHIS
13   HISHISHISHIS

Samples:

sample_1: alpha sub-domain, [U-98% 13C; U-98% 15N], 0.5 mM; sodium phosphate 50 mM; sodium chloride 100 mM; DTT 5 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

GB AAO43974.1 AAO43974
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks