BMRB Entry 18601

Title:
GGBP Ecoli unliganded solution structure
Deposition date:
2012-07-19
Original release date:
2012-08-27
Authors:
Millet, Oscar; Castano, David; Ortega, Gabriel
Citation:

Citation: Millet, Oscar; Ortega, Gabriel; Castano, David; Ortega, Tammo. "GGBP free solution structure"  .

Assembly members:

Assembly members:
GGBP_unbound, polymer, 310 residues, 33551.020 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-11d

Data sets:
Data typeCount
13C chemical shifts542
15N chemical shifts281
1H chemical shifts281

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GGBP_unbound1

Entities:

Entity 1, GGBP_unbound 310 residues - 33551.020 Da.

1   METALAASPTHRARGILEGLYVALTHRILE
2   TYRLYSTYRASPASPASNPHEMETSERVAL
3   VALARGLYSALAILEGLUGLNASPALALYS
4   ALAALAPROASPVALGLNLEULEUMETASN
5   ASPSERGLNASNASPGLNSERLYSGLNASN
6   ASPGLNILEASPVALLEULEUALALYSGLY
7   VALLYSALALEUALAILEASNLEUVALASP
8   PROALAALAALAGLYTHRVALILEGLULYS
9   ALAARGGLYGLNASNVALPROVALVALPHE
10   PHEASNLYSGLUPROSERARGLYSALALEU
11   ASPSERTYRASPLYSALATYRTYRVALGLY
12   THRASPSERLYSGLUSERGLYILEILEGLN
13   GLYASPLEUILEALALYSHISTRPALAALA
14   ASNGLNGLYTRPASPLEUASNLYSASPGLY
15   GLNILEGLNPHEVALLEULEULYSGLYGLU
16   PROGLYHISPROASPALAGLUALAARGTHR
17   THRTYRVALILELYSGLULEUASNASPLYS
18   GLYILELYSTHRGLUGLNLEUGLNLEUASP
19   THRALAMETTRPASPTHRALAGLNALALYS
20   ASPLYSMETASPALATRPLEUSERGLYPRO
21   ASNALAASNLYSILEGLUVALVALILEALA
22   ASNASNASPALAMETALAMETGLYALAVAL
23   GLUALALEULYSALAHISASNLYSSERSER
24   ILEPROVALPHEGLYVALASPALALEUPRO
25   GLUALALEUALALEUVALLYSSERGLYALA
26   LEUALAGLYTHRVALLEUASNASPALAASN
27   ASNGLNALALYSALATHRPHEASPLEUALA
28   LYSASNLEUALAASPGLYLYSGLYALAALA
29   ASPGLYTHRASNTRPLYSILEASPASNLYS
30   VALVALARGVALPROTYRVALGLYVALASP
31   LYSASPASNLEUALAGLUPHESERLYSLYS

Samples:

sample_assi: GGBP_unbound, [U-13C; U-15N; U-2H], 1 mM; sodium azide 1 mM; sodium chloride 150 mM; calcium chloride 10 uM; TRIS 20 mM; H2O 90%; D2O 10%

sample_rdc: GGBP_unbound, [U-15N], 1 mM; sodium azide 1 mM; sodium chloride 150 mM; calcium chloride 10 uM; TRIS 20 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.150 M; pH: 7; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_assiisotropicsample_conditions_1
3D HNCO - editedsample_assiisotropicsample_conditions_1
3D HNCA - editedsample_assiisotropicsample_conditions_1
3D HN(coca)CB - editedsample_assiisotropicsample_conditions_1
3D HN(CO)CA - editedsample_assiisotropicsample_conditions_1
3D HNCB - editedsample_assiisotropicsample_conditions_1
2D 1H-15N HSQC - editedsample_rdcisotropicsample_conditions_1
2D 1H-15N HSQC - editedsample_rdcisotropicsample_conditions_1
3D HNCO - editedsample_rdcisotropicsample_conditions_1
3D HNCO - editedsample_rdcisotropicsample_conditions_1

Software:

TOPSPIN v2, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, peak picking

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Bruker INOVA 800 MHz
  • Bruker INOVA 600 MHz

Related Database Links:

BMRB 18609
PDB
DBJ BAB36465 BAE76627 BAG77941 BAI26274 BAI31400
EMBL CAA29132 CAP76652 CAQ89737 CAQ99072 CAR03578
GB AAA24169 AAA60523 AAC75211 AAG57288 AAN43756
PRF 2014253AU
REF NP_311069 NP_416655 NP_708049 WP_001035789 WP_001036935
SP P0AEE5 P0AEE6 P0AEE7
AlphaFold P0AEE5 P0AEE6 P0AEE7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks