BMRB Entry 18527

Title:
1H chemical shifts for the C-terminal beta-tubulin 394-455 fragment
Deposition date:
2012-06-16
Original release date:
2012-07-13
Authors:
Jimenez, M. Angeles; Andreu, Jose
Citation:

Citation: Andreu, M. Angeles; Evangelio, Juan; Aranda, Carlos; Lopez-Brauet, Adamari; Andreu, David; Rico, Manuel; Lagos, Rosalba; Andreu, Jose; Monasterio, Octavio. "Helicity of alpha(404-451) and bet(394-445) tubulin C-terminal recombinant peptides"  Protein Sci. 8, 788-799 (1999).
PubMed: 10211825

Assembly members:

Assembly members:
beta_tubulin_394-445, polymer, 58 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pT7-7

Entity Sequences (FASTA):

Entity Sequences (FASTA):
beta_tubulin_394-445: ARIRAPFLHWYTGEGMDEME FTEAESNMNDLVSEYQQYQD ATADEQGEFEEEGEEDEA

Data sets:
Data typeCount
1H chemical shifts340

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1beta tubulin 394-4451

Entities:

Entity 1, beta tubulin 394-445 58 residues - Formula weight is not available

ARIRAP is the N-terminal cloning tag

1   ALAARGILEARGALAPROPHELEUHISTRP
2   TYRTHRGLYGLUGLYMETASPGLUMETGLU
3   PHETHRGLUALAGLUSERASNMETASNASP
4   LEUVALSERGLUTYRGLNGLNTYRGLNASP
5   ALATHRALAASPGLUGLNGLYGLUPHEGLU
6   GLUGLUGLYGLUGLUASPGLUALA

Samples:

sample_1: beta tubulin 394-445 2 mM; H2O 63 v/v; D2O 7 v/v; TFE, [U-99% 2H], 30 v/v; TSP 0.2 mM

sample_conditions_1: ionic strength: 0 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - processing

NMR spectrometers:

  • Bruker AMX 600 MHz