BMRB Entry 18513

Title:
RECOMBINANT TAMAPIN NMR solution structure.
Deposition date:
2012-06-08
Original release date:
2013-12-09
Authors:
del Rio-Portilla, Federico; Ramirez-Cordero, Belen
Citation:

Citation: Ramirez-Cordero, B.; Flores-Solis, D.; Saucedo, A.; Hernandez-Lupez, R.; Cano-Sanchez, P.; Chavez-Uribe, I.; del Rio-Portilla, F.. "Structural change due to deletion of two residues in GS-tamapin"  to be published ., .-..

Assembly members:

Assembly members:
recombinant-tamapin, polymer, 31 residues, 3470.188 Da.

Natural source:

Natural source:   Common Name: Indian red scorpion   Taxonomy ID: 34647   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mesobuthus tamulus

Experimental source:

Experimental source:   Production method: purified from natural source   Host organism: Escherichia coli   Vector: pET32a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
recombinant-tamapin: AFCNLRRCELSCRSLGLLGK CIGEECKCVPY

Data sets:
Data typeCount
1H chemical shifts205

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1recombinant-tamapin1

Entities:

Entity 1, recombinant-tamapin 31 residues - 3470.188 Da.

1   ALAPHECYSASNLEUARGARGCYSGLULEU
2   SERCYSARGSERLEUGLYLEULEUGLYLYS
3   CYSILEGLYGLUGLUCYSLYSCYSVALPRO
4   TYR

Samples:

sample: recombinant-tamapin 0.8 mM; H2O 95%; D2O 5%

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
DQFCOSYsampleisotropicsample_conditions_1
TOCSYsampleisotropicsample_conditions_1
NOESYsampleisotropicsample_conditions_1

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMR spectrometers:

  • Varian Inova 500 MHz

Related Database Links:

BMRB 17227
PDB
SP P59869
AlphaFold P59869