BMRB Entry 18494

Title:
Assignment of the -Helical Membrane Protein TM0026 from Thermotoga maritima.
Deposition date:
2012-05-30
Original release date:
2013-01-03
Authors:
Kroncke, Brett; Columbus, Linda
Citation:

Citation: Kroncke, Brett; Columbus, Linda. "Backbone 1H, 13C and 15N resonance assignments of the -helical membrane protein TM0026 from Thermotoga maritima."  Biomol. NMR Assignments 7, 203-206 (2013).
PubMed: 23011877

Assembly members:

Assembly members:
TM0026, polymer, 80 residues, 9576.3 Da.

Natural source:

Natural source:   Common Name: thermotogales   Taxonomy ID: 2336   Superkingdom: Bacteria   Kingdom: Thermotoga   Genus/species: Thermotoga Maritima

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET25b

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts171
15N chemical shifts59
1H chemical shifts59

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TM0026 monomer1

Entities:

Entity 1, TM0026 monomer 80 residues - 9576.3 Da.

Residues 1-12 represent a non-native affinity tag

1   METGLYSERASPLYSILEHISHISHISHIS
2   HISHISMETPHETHRLYSALAVALLEUSER
3   ILEPHESERTRPALALEUVALLEUGLULEU
4   ILEVALLEUPHETYRTYRLEUTRPARGGLY
5   LEUARGPROVALGLUPHETYRLEUASNLEU
6   GLYLEULEUGLYLEUTHRVALPROPHELEU
7   VALPHELEUVALVALARGGLULYSLYSLYS
8   ARGARGGLUGLUASPASPGLYLYSASPARG

Samples:

sample_1: TM0026, [U-100% 13C]; [U-100% 15N]; [U-95% 2H], 500 ± 100 mM; H2O 90 ± 100 %; D2O 10 ± 100 %

sample_conditions_1: pH: 6.2; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.0.3, Bruker Biospin, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Keller and Wuthrich - chemical shift assignment, collection, processing

NMR spectrometers:

  • Bruker AMX 600 MHz
  • Bruker AMX 800 MHz

Related Database Links:

REF NP_227842 NP_227842 WP_004082479 WP_011943474 WP_012896089
GB AAD35120 ABQ46915 ACB09270 ADA66753 AGL48949

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks