BMRB Entry 18390

Title:
Chemical Shift Assignment and Solution Structure of ChR145 from Drosophila melanogaster. Northeast Structural Genomics Consortium Target Fr822A
Deposition date:
2012-04-11
Original release date:
2012-05-15
Authors:
Lee, Hsiau-Wei; Lee, Dan; Kohan, Eitan; Janjua, Haleema; Xiao, Rong; Acton, Thomas; Everett, John; Montelione, Gaetano; Prestegard, James
Citation:

Citation: Lee, Hsiau-Wei. "Solution Structure of Fr822A from Drosophila melanogaster."  .

Assembly members:

Assembly members:
Fr822A, polymer, 170 residues, 20032.592 Da.

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15Nano6HT_NESG

Data sets:
Data typeCount
13C chemical shifts321
15N chemical shifts78
1H chemical shifts494

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Fr822A1

Entities:

Entity 1, Fr822A 170 residues - 20032.592 Da.

1   METASNTYRSERTHRGLYTHRASPALAASN
2   THRLEUPHEVALASPGLYGLUARGVALLEU
3   CYSPHEHISGLYPROLEUILETYRGLUALA
4   LYSVALLEULYSTHRLYSPROASPALATHR
5   PROVALGLUTYRTYRILEHISTYRALAGLY
6   TRPSERLYSASNTRPASPGLUTRPVALPRO
7   GLUASNARGVALLEULYSTYRASNASPASP
8   ASNVALLYSARGARGGLNGLULEUALAARG
9   GLNCYSGLYGLUARGMETASNTYRSERTHR
10   GLYTHRASPALAASNTHRLEUPHEVALASP
11   GLYGLUARGVALLEUCYSPHEHISGLYPRO
12   LEUILETYRGLUALALYSVALLEULYSTHR
13   LYSPROASPALATHRPROVALGLUTYRTYR
14   ILEHISTYRALAGLYTRPSERLYSASNTRP
15   ASPGLUTRPVALPROGLUASNARGVALLEU
16   LYSTYRASNASPASPASNVALLYSARGARG
17   GLNGLULEUALAARGGLNCYSGLYGLUARG

Samples:

NC_Sample: Fr822A, [U-100% 13C; U-100% 15N], 0.9 mM; sodium phosphate 20 mM; sodium chloride 200 mM; DTT 5 mM

Phage: Fr822A, [U-100% 15N], 0.9 mM; sodium phosphate 20 mM; sodium chloride 200 mM; Pf1 phage 12 mg; DTT 5 mM

C12E5: Fr822A, [U-100% 15N], 0.9 mM; sodium phosphate 20 mM; sodium chloride 200 mM; C12E5 4.2%; DTT 5 mM

Fr822A-NMR: ionic strength: 200 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCNC_SampleisotropicFr822A-NMR
2D 1H-13C HSQC aliphaticNC_SampleisotropicFr822A-NMR
2D 1H-13C HSQC aromaticNC_SampleisotropicFr822A-NMR
3D CBCA(CO)NHNC_SampleisotropicFr822A-NMR
3D C(CO)NHNC_SampleisotropicFr822A-NMR
3D HNCONC_SampleisotropicFr822A-NMR
3D HNCANC_SampleisotropicFr822A-NMR
3D HNCACBNC_SampleisotropicFr822A-NMR
3D HBHA(CO)NHNC_SampleisotropicFr822A-NMR
3D HCCH-TOCSYNC_SampleisotropicFr822A-NMR
3D 1H-15N NOESYNC_SampleisotropicFr822A-NMR
3D 1H-13C NOESY aliphaticNC_SampleisotropicFr822A-NMR
3D 1H-13C NOESY aromaticNC_SampleisotropicFr822A-NMR
2D 1H-15N NH-J-Modulation-HSQCNC_SampleisotropicFr822A-NMR
2D 1H-15N NH-J-Modulation-HSQCPhageanisotropicFr822A-NMR
2D 1H-15N NH-J-Modulation-HSQCC12E5anisotropicFr822A-NMR

Software:

SPARKY v3.14, Goddard - chemical shift assignment, data analysis

VNMRJ v2.1B, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

X-PLOR NIH v2.18, Schwieters, Kuszewski, Tjandra and Clore - refinement

PSVS, Bhattacharya and Montelione - data analysis

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks