BMRB Entry 18185

Title:
Backbone 1H and 15N Chemical Shift Assignments of the VHS-UIM domains of STAM2
Deposition date:
2012-01-06
Original release date:
2012-04-05
Authors:
Walker, Olivier; Lange, Anja; Castaneda, Carlos; Hoeller, Daniela; Lancelin, Jean-Marc; Fushman, David
Citation:

Citation: Lange, Anja; Castaneda, Carlos; Hoeller, Daniela; Lancelin, Jean-Marc; Fushman, David; Walker, Olivier. "Evidence for cooperative and domain-specific binding of the signal transducing adaptor molecule 2 (STAM2) to Lys63-linked diubiquitin."  J. Biol. Chem. 287, 18687-18699 (2012).
PubMed: 22493438

Assembly members:

Assembly members:
VHS-UIM_domains_of_STAM2, polymer, 192 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETEM-60

Data sets:
Data typeCount
15N chemical shifts165
1H chemical shifts165

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1VHS-UIM1

Entities:

Entity 1, VHS-UIM 192 residues - Formula weight is not available

1   GLYALAMETGLYMETPROLEUPHETHRALA
2   ASNPROPHEGLUGLNASPVALGLULYSALA
3   THRASNGLUTYRASNTHRTHRGLUASPTRP
4   SERLEUILEMETASPILECYSASPLYSVAL
5   GLYSERTHRPROASNGLYALALYSASPCYS
6   LEULYSALAILEMETLYSARGVALASNHIS
7   LYSVALPROHISVALALALEUGLNALALEU
8   THRLEULEUGLYALACYSVALALAASNCYS
9   GLYLYSILEPHEARGLEUGLYVALCYSSER
10   ARGASPPHEALATHRGLUVALARGALAVAL
11   ILELYSASNLYSALAHISPROLYSVALCYS
12   GLULYSLEULYSSERLEUMETVALGLUTRP
13   SERGLUGLUPHEGLNLYSASPPROGLNPHE
14   SERLEUILESERALATHRILELYSSERMET
15   LYSGLUGLUGLYILETHRPHEPROPROALA
16   GLYSERGLNTHRVALSERALAALAALALYS
17   ASNGLYTHRSERSERASNLYSASNLYSGLU
18   ASPGLUASPILEALALYSALAILEGLULEU
19   SERLEUGLNGLUGLNLYSGLNGLNHISTHR
20   GLUTHR

Samples:

sample_1: VHS-UIM domains of STAM2, [U-13C; U-15N], 200-400 uM; sodium phosphate 20 mM; sodium azide 0.02%

sample_conditions_1: ionic strength: 20 mM; pH: 6.8; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

CARA, Keller and Wuthrich - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 10264
PDB
DBJ BAF84954 BAF85536 BAG72771 BAK62690
EMBL CAB63735 CAL38297
GB AAC63963 AAC63964 AAH28740 AAI12605 AAY14712
REF NP_001069574 NP_001267210 NP_005834 XP_002749486 XP_002926244
SP O75886
TPG DAA32691
AlphaFold O75886

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks