BMRB Entry 18160

Title:
Solution NMR structure of the C-terminal extension of two bacterial light, oxygen, voltage (LOV) photoreceptor proteins from Pseudomonas putida
Deposition date:
2011-12-23
Original release date:
2013-07-08
Authors:
Rani, Raj; Lecher, Justin; Hartmann, Rudolf; Krauss, Ulrich; Jaeger, Karl-Erich; Willbold, Dieter
Citation:

Citation: Rani, Raj; Jentzsch, Katrin; Lecher, Justin; Hartmann, Rudolf; Willbold, Dieter; Jaeger, Karl-Erich; Krauss, Ulrich. "Conservation of Dark Recovery Kinetic Parameters and Structural Features in the Pseudomonadaceae "Short" Light, Oxygen, Voltage (LOV) Protein Family: Implications for the Design of LOV-Based Optogenetic Tools"  Biochemistry 52, 4460-4473 (2013).
PubMed: 23746326

Assembly members:

Assembly members:
AceSB2Pep, polymer, 30 residues, 3420.7601 Da.

Natural source:

Natural source:   Common Name: Pseudomonas putida   Taxonomy ID: 303   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas putida

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
AceSB2Pep: XTAQVFAEERVRELEAEVAE LRRQQGQAKH

Data sets:
Data typeCount
1H chemical shifts214

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AceSB2Pep1

Entities:

Entity 1, AceSB2Pep 30 residues - 3420.7601 Da.

1   ACETHRALAGLNVALPHEALAGLUGLUARG
2   VALARGGLULEUGLUALAGLUVALALAGLU
3   LEUARGARGGLNGLNGLYGLNALALYSHIS

Samples:

PpSB2-Jalpha: AceSB2Pep 1.0 mM; sodium phosphate 100.0 mM; sodium chloride 50.0 mM; 2,2,2-Trifluoroethanol 25.0%; H2O 65.0%; deuterium oxide 10.0%

Standard: ionic strength: 150.0 mM; pH: 6.300; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYPpSB2-JalphaisotropicStandard

Software:

ANALYSIS v2.2, CCPN - assignment, data evaluation

NMRPipe v7.3.2012.114.11.33, NIH - conversion, processing

VNMRJ v2.3A, Agilent Technologies (formerly Varian) - data recording, spectrometer operation

NMR spectrometers:

  • Varian VNMRS 900 MHz

Related Database Links:

UniProt Q88JB0
PDB
GB AAN68347 AEV23113 KMU97968 KMY35015
REF NP_744883 WP_010953652 WP_029615462
AlphaFold Q88JB0