BMRB Entry 18020

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments of Apo Human Integrin Alpha1 I-domain
Deposition date:
2011-10-25
Original release date:
2013-02-19
Authors:
Chin, Yanni; Headey, Stephen; McEwan, Paul; Emsley, Jonas; Simpson, Jamie; Scanlon, Martin
Citation:

Citation: Chin, Yanni K-Y; Headey, Stephen; Mohanty, Biswaranjan; Emsley, Jonas; Simpson, Jamie; Scanlon, Martin. "Assignments of human integrin 1I domain in the apo and Mg+ bound states."  Biomol. NMR Assignments 8, 117-121 (2014).
PubMed: 23339031

Assembly members:

Assembly members:
Integrin_Alpha1_I-domain, polymer, 192 residues, 21485 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: p28a+

Data sets:
Data typeCount
13C chemical shifts523
15N chemical shifts173
1H chemical shifts173

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
11 I-domain1

Entities:

Entity 1, 1 I-domain 192 residues - 21485 Da.

1   SERTHRGLNLEUASPILEVALILEVALLEU
2   ASPGLYSERASNSERILETYRPROTRPASP
3   SERVALTHRALAPHELEUASNASPLEULEU
4   GLUARGMETASPILEGLYPROLYSGLNTHR
5   GLNVALGLYILEVALGLNTYRGLYGLUASN
6   VALTHRHISGLUPHEASNLEUASNLYSTYR
7   SERSERTHRGLUGLUVALLEUVALALAALA
8   LYSLYSILEVALGLNARGGLYGLYARGGLN
9   THRMETTHRALALEUGLYILEASPTHRALA
10   ARGLYSGLUALAPHETHRGLUALAARGGLY
11   ALAARGARGGLYVALLYSLYSVALMETVAL
12   ILEVALTHRASPGLYGLUSERHISASPASN
13   HISARGLEULYSLYSVALILEGLNASPCYS
14   GLUASPGLUASNILEGLNARGPHESERILE
15   ALAILELEUGLYSERTYRASNARGGLYASN
16   LEUSERTHRGLULYSPHEVALGLUGLUILE
17   LYSSERILEALASERGLUPROTHRGLULYS
18   HISPHEPHEASNVALSERASPGLULEUALA
19   LEUVALTHRILEVALLYSTHRLEUGLYGLU
20   ARGILE

Samples:

1H_13C_15N_Alpha1I: Integrin Alpha1 I-domain, [U-99% 13C; U-99% 15N], 0.4 mM; sodium phosphate 50 mM; sodium chloride 100 mM; H2O 90%; D2O 10%

Apo_Condition: pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC1H_13C_15N_Alpha1IisotropicApo_Condition
3D CBCA(CO)NH1H_13C_15N_Alpha1IisotropicApo_Condition
3D HNCA1H_13C_15N_Alpha1IisotropicApo_Condition
3D HNCO1H_13C_15N_Alpha1IisotropicApo_Condition
3D HNCACB1H_13C_15N_Alpha1IisotropicApo_Condition
3D HCACO1H_13C_15N_Alpha1IisotropicApo_Condition
3D 1H-15N NOESY1H_13C_15N_Alpha1IisotropicApo_Condition

Software:

TOPSPIN, Bruker Biospin - processing

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 18021 18942
PDB
DBJ BAG62150
GB AAI37122 AAI37123 EAW54866 EAW54867 EAW54868
REF NP_852478 XP_001094788 XP_002815595 XP_003276564 XP_003827398
SP P56199
AlphaFold P56199

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks