BMRB Entry 17864

Title:
1H, 13C, and 15N Chemical Shift Assignments for human FAIM-CTD
Deposition date:
2011-08-16
Original release date:
2011-08-31
Authors:
Ma, Shuaipeng; Li, Guoming; Zheng, Xiaofeng; Jin, Changwen
Citation:

Citation: Ma, Shuaipeng; Jin, Changwen. "1H, 13C and 15N Assigned Chemical Shifts for human FAIM CTD"  .

Assembly members:

Assembly members:
FAIM-CTD_polypeptide, polymer, 89 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a(+)

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts356
15N chemical shifts92
1H chemical shifts565

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CTD1

Entities:

Entity 1, CTD 89 residues - Formula weight is not available

1   GLUASPARGSERLYSTHRTHRASNTHRTRP
2   VALLEUHISMETASPGLYGLUASNPHEARG
3   ILEVALLEUGLULYSASPALAMETASPVAL
4   TRPCYSASNGLYLYSLYSLEUGLUTHRALA
5   GLYGLUPHEVALASPASPGLYTHRGLUTHR
6   HISPHESERILEGLYASNHISASPCYSTYR
7   ILELYSALAVALSERSERGLYLYSARGLYS
8   GLUGLYILEILEHISTHRLEUILEVALASP
9   ASNARGGLUILEPROGLUILEALASER

Samples:

sample_1: FAIM-CTD polypeptide, [U-13C; U-15N], 0.5 – 1.0 mM; H20 90%; D2O 10%

sample_conditions_1: ionic strength: 0.05 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAA91695
EMBL CAG33403
GB AAH12478 ABM82508 ABM85702 AIC51733 EAW79057
REF NP_001028202 NP_001028203 NP_001028204 NP_060617 XP_001157090
SP Q9NVQ4
AlphaFold Q9NVQ4

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks