BMRB Entry 17620

Title:
1H,15N and 13C Assignment of the D2 domain of the Human Fibroblast Growth Factor Receptor 4
Deposition date:
2011-05-03
Original release date:
2011-06-03
Authors:
Elshorst, Bettina; Saxena, Krishna; Schieborr, Ulrich; Schwalbe, Harald
Citation:

Citation: Elshorst, Bettina; Saxena, Krishna; Schieborr, Ulrich; Schwalbe, Harald. "1H, 13C and 15N assignment of D2 domain of human fibroblast growth factor receptor 4."  Biomol. NMR Assignments 7, 179-182 (2013).
PubMed: 22752790

Assembly members:

Assembly members:
FGFR4_D2, polymer, 114 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: BL21(DE3)

Data sets:
Data typeCount
13C chemical shifts343
15N chemical shifts87
1H chemical shifts546

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FGFR4 D21

Entities:

Entity 1, FGFR4 D2 114 residues - Formula weight is not available

1   METASNARGHISSERTYRPROGLNGLNALA
2   PROTYRTRPTHRHISPROGLNARGMETGLU
3   LYSLYSLEUHISALAVALPROALAGLYASN
4   THRVALLYSPHEARGCYSPROALAALAGLY
5   ASNPROTHRPROTHRILEARGTRPLEULYS
6   ASPGLYGLNALAPHEHISGLYGLUASNARG
7   ILEGLYGLYILEARGLEUARGHISGLNHIS
8   TRPSERLEUVALMETGLUSERVALVALPRO
9   SERASPARGGLYTHRTYRTHRCYSLEUVAL
10   GLUASNALAVALGLYSERILEARGTYRASN
11   TYRLEULEUASPVALLEULEUGLUHISHIS
12   HISHISHISHIS

Samples:

sample_1: FGFR4 D2, [U-99% 13C; U-99% 15N], 0.7 mM; BIS-TRIS 25 mM; sodium chloride 200 mM; arginine 25 mM; TSP 1 mM; H2O 90%; D2O 10%

sample_2: FGFR4 D2, [U-99% 13C; U-99% 15N], 0.7 mM; BIS-TRIS 25 mM; sodium chloride 200 mM; arginine 25 mM; TSP 1 mM; D2O 100%

sample_conditions_1: ionic strength: 200 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HMQCsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D CCH-TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1

Software:

CARA, Rochus Keller - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DRX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks