BMRB Entry 17606

Title:
Solution structure of RBBP1 chromobarrel domain
Deposition date:
2011-04-28
Original release date:
2012-02-06
Authors:
Gong, Weibin; Feng, Yingang
Citation:

Citation: Gong, Weibin; Zhou, Tao; Mo, Jinjin; Perrett, Sarah; Wang, Jinfeng; Feng, Yingang. "Structural Insight into Recognition of Methylated Histone Tails by Retinoblastoma-binding Protein 1."  J. Biol. Chem. 287, 8531-8540 (2012).
PubMed: 22247551

Assembly members:

Assembly members:
entity, polymer, 76 residues, 9082.347 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET30a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts327
15N chemical shifts73
1H chemical shifts476

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RBBP11

Entities:

Entity 1, RBBP1 76 residues - 9082.347 Da.

1   GLUASPMETGLUPROCYSLEUTHRGLYTHR
2   LYSVALLYSVALLYSTYRGLYARGGLYLYS
3   THRGLNLYSILETYRGLUALASERILELYS
4   SERTHRGLUILEASPASPGLYGLUVALLEU
5   TYRLEUVALHISTYRTYRGLYTRPASNVAL
6   ARGTYRASPGLUTRPVALLYSALAASPARG
7   ILEILETRPPROLEUASPLYSGLYLEUGLU
8   HISHISHISHISHISHIS

Samples:

sample_1: RBBP1, [U-95% 15N], 0.5 mM; sodium phosphate 50 mM; sodium chloride 50 mM; DTT 5 mM; H2O 90%; D2O 10%

sample_2: entity, [U-95% 13C; U-95% 15N], 0.5 mM; sodium phosphate 50 mM; sodium chloride 50 mM; DTT 5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.4 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_1
3D HCACOsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

PDB
GB AAB25833 AAB25834 AAB25835 AAB28543 AAI72363
REF NP_001101499 NP_001231108 NP_001243510 NP_002883 NP_075376
SP P29374
TPG DAA25277
AlphaFold P29374

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks