BMRB Entry 17598

Title:
Solution Structure of PilP from Pseudomonas aeruginosa
Deposition date:
2011-04-22
Original release date:
2011-12-20
Authors:
Howell, P. Lynne; Tammam, Stephanie; Chong, P. Andrew; Forman-Kay, Julie
Citation:

Citation: Tammam, Stephanie; Sampaleanu, Liliana; Koo, Jason; Sundaram, Priyanka; Ayers, Melissa; Chong, P. Andrew; Forman-Kay, Julie; Burrows, Lori; Howell, P. Lynne. "Characterization of the PilN, PilO and PilP type IVa pilus subcomplex"  Mol. Micro. 82, 1496-1514 (2011).
PubMed: 22053789

Assembly members:

Assembly members:
PilP, polymer, 111 residues, 12427.167 Da.

Natural source:

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data sets:
Data typeCount
13C chemical shifts367
15N chemical shifts107
1H chemical shifts741

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PilP1

Entities:

Entity 1, PilP 111 residues - 12427.167 Da.

1   ASPLEUTHRVALARGGLNLYSGLYASNLYS
2   VALILELYSPROASPGLUTHRARGVALLYS
3   GLNPHELEUGLUGLYPHEASNILEGLUTHR
4   PHEGLUMETVALGLYTHRLEUSERASNALA
5   GLNGLYTHRPHEALALEUVALLYSGLYALA
6   GLYGLYVALHISARGVALARGVALGLYASP
7   TYRLEUGLYARGASNASPGLYLYSVALVAL
8   GLYILESERGLUGLYLYSILEASPVALILE
9   GLUILEVALPROASPGLYGLUGLYASNTRP
10   LEUGLUARGPROARGSERLEUTHRLEULYS
11   GLUARGSERLEUGLUHISHISHISHISHIS
12   HIS

Samples:

sample_1: sodium chloride 150 mM; TRIS 20 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.15 M; pH: 7.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCC-TOCSYsample_1isotropicsample_conditions_1
3D CCC-TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

TALOS, Cornilescu, Delaglio and Bax - geometry optimization

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAK87361 BAP24857 BAP53627 BAQ42890 BAR70506
EMBL CAW30185 CCQ86063 CDH73786 CDH80106 CDI93614
GB AAA87405 AAG08426 ABJ14425 ABR86649 AEO77634
REF NP_253728 WP_003095836 WP_003116136 WP_012077721 WP_021263517

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks