BMRB Entry 17589

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17589

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Backbone Resonance Assignments for Prp24-RRM3
Deposition date:
2011-04-12
Original release date:
2011-06-22
Authors:
Martin-Tumasz, Stephen; Butcher, Samuel
Citation:

Citation: Martin-Tumasz, Stephen; Richie, Ashley; Clos, Lawrence; Brow, David; Butcher, Samuel. "A novel occluded RNA recognition motif in Prp24 unwinds the U6 RNA internal stem loop."  Nucleic Acids Res. 39, 7837-7847 (2011).
PubMed: 21653550

Assembly members:

Assembly members:
RRM3, polymer, 93 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-21b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RRM3: MEGREIMIRNLSTELLDENL LRESFEGFGSIEKINIPAGQ KEHSFNNCCAFMVFENKDSA ERALQMNRSLLGNREISVSL ADKKPLWHHHHHH

Data sets:
Data typeCount
13C chemical shifts253
15N chemical shifts84
1H chemical shifts84

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RRM31

Entities:

Entity 1, RRM3 93 residues - Formula weight is not available

1   METGLUGLYARGGLUILEMETILEARGASN
2   LEUSERTHRGLULEULEUASPGLUASNLEU
3   LEUARGGLUSERPHEGLUGLYPHEGLYSER
4   ILEGLULYSILEASNILEPROALAGLYGLN
5   LYSGLUHISSERPHEASNASNCYSCYSALA
6   PHEMETVALPHEGLUASNLYSASPSERALA
7   GLUARGALALEUGLNMETASNARGSERLEU
8   LEUGLYASNARGGLUILESERVALSERLEU
9   ALAASPLYSLYSPROLEUTRPHISHISHIS
10   HISHISHIS

Samples:

13C15N_H2O: RRM3, [U-99% 13C; U-99% 15N], 600 uM; potassium phosphate pH 6 18 mM; potassium chloride 45 mM; DTT 0.9 mM; H2O 90%; D2O 10%

Reference: RRM3, [U-99% 13C; U-99% 15N], 600 uM; potassium phosphate pH 6 18 mM; potassium chloride 45 mM; DSS 1 uM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC13C15N_H2Oisotropicsample_conditions_1
3D CBCA(CO)NH13C15N_H2Oisotropicsample_conditions_1
3D HNCACB13C15N_H2Oisotropicsample_conditions_1
3D HNCO13C15N_H2Oisotropicsample_conditions_1
2D 1H-13C HSQC aliphaticReferenceisotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Varian Uniform NMR System 900 MHz

Related Database Links:

BMRB 16243 16244
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks