BMRB Entry 17547

Title:
Hevein-type Antifungal Peptide with a Unique 10-Cysteine Motif
Deposition date:
2011-03-23
Original release date:
2011-10-26
Authors:
Balashova, Tamara; Vassilevski, Alexander; Odintsova, Tatyana; Grishin, Eugene; Egorov, Tsezi; Arseniev, Alexander
Citation:

Citation: Dubovskii, Peter; Vassilevski, Alexander; Slavokhotova, Anna; Odintsova, Tatyana; Grishin, Eugene; Egorov, Tsezi; Arseniev, Alexander. "Solution structure of a defense peptide from wheat with a 10-cysteine motif."  Biochem. Biophys. Res. Commun. 411, 14-18 (2011).
PubMed: 21704019

Assembly members:

Assembly members:
Hevein-type Antifungal Peptide with a Unique 10-Cysteine Motif, polymer, 44 residues, 4450.046 Da.

Natural source:

Natural source:   Common Name: wheat   Taxonomy ID: 376535   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Triticum kiharae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-32b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Hevein-type Antifungal Peptide with a Unique 10-Cysteine Motif: AQRCGDQARGAKCPNCLCCG KYGFCGSGDAYCGAGSCQSQ CRGC

Data sets:
Data typeCount
1H chemical shifts243

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Hevein-type Antifungal Peptide with a Unique 10-Cysteine Motif1

Entities:

Entity 1, Hevein-type Antifungal Peptide with a Unique 10-Cysteine Motif 44 residues - 4450.046 Da.

1   ALAGLNARGCYSGLYASPGLNALAARGGLY
2   ALALYSCYSPROASNCYSLEUCYSCYSGLY
3   LYSTYRGLYPHECYSGLYSERGLYASPALA
4   TYRCYSGLYALAGLYSERCYSGLNSERGLN
5   CYSARGGLYCYS

Samples:

sample_1: entity 0.5 ± 0.05 mM; H2O 90%; D2O 10%

sample_2: entity 0.5 ± 0.05 mM; D2O 100%

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

TOPSPIN v2.2, BRUKER - collection, processing

XEASY, Bartels et al. - chemical shift assignment, chemical shift calculation, peak picking

CYANA v2.0, Guntert, Mumenthaler and Wuthrich - structure solution

Molmol v2.1-2.6, Koradi, Billeter and Wuthrich - visualization

NMR spectrometers:

  • Bruker Avance-1 700 MHz

Related Database Links:

PDB
EMBL CCD30733 CCD30734 CCD30735 CCD30736 CCD30738
SP P85966
AlphaFold P85966