BMRB Entry 17517

Title:
Pseudouridine_ASL_Tyr
Deposition date:
2011-03-09
Original release date:
2011-08-02
Authors:
Denmon, Andria; Wang, Jiachen; Nikonowicz, Edward
Citation:

Citation: Denmon, Andria; Wang, Jiachen; Nikonowicz, Edward. "Conformation effects of base modification on the anticodon stem-loop of Bacillus subtilis tRNA(Tyr)."  J. Mol. Biol. 412, 285-303 (2011).
PubMed: 21782828

Assembly members:

Assembly members:
tRNATYR, polymer, 17 residues, 324.183 Da.

Natural source:

Natural source:   Common Name: B. subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
tRNATYR: GGGGACUGUAAAXCCCC

Data sets:
Data typeCount
13C chemical shifts35
1H chemical shifts80
31P chemical shifts16

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1tRNATYR1

Entities:

Entity 1, tRNATYR 17 residues - 324.183 Da.

1   GGGGACUGUA
2   AAPSUCCCC

Samples:

sample_1: Pseudouridine_ASL_Tyr 2.0 mM; potassium chloride 10 mM; potassium phosphate 10 mM; EDTA 0.02 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D Natural Abundance HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 31P-1Hsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH v2.19, Schwieters, Kuszewski, Tjandra and Clore - refinement

FELIX, Accelrys Software Inc. - chemical shift assignment, peak picking, processing

InsightII, Accelrys Software Inc. - data analysis

VNMR, Varian - collection

NMR spectrometers:

  • Varian INOVA 500 MHz