BMRB Entry 17510

Title:
1H, 13C and 15N backbone and side-chain resonance assignments of Drosophila melanogaster Ssu72
Deposition date:
2011-03-04
Original release date:
2011-07-07
Authors:
Werner-Allen, Jon; Zhou, Pei
Citation:

Citation: Werner-Allen, Jon; Zhou, Pei. "(1)H, (13)C and (15)N backbone and side-chain resonance assignments of Drosophila melanogaster Ssu72."  Biomol. NMR Assignments 6, 57-61 (2012).
PubMed: 21732054

Assembly members:

Assembly members:
Ssu72, polymer, 198 residues, Formula weight is not available
PHOSPHATE ION, non-polymer, 94.971 Da.

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet15b

Data sets:
Data typeCount
13C chemical shifts791
15N chemical shifts177
1H chemical shifts1287

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Drosophila melanogaster Ssu721
2inorganic phosphate2

Entities:

Entity 1, Drosophila melanogaster Ssu72 198 residues - Formula weight is not available

1   GLYSERHISMETTHRASPPROSERLYSLEU
2   ALAVALALAVALVALCYSSERSERASNMET
3   ASNARGSERMETGLUALAHISASNPHELEU
4   ALALYSLYSGLYPHEASNVALARGSERTYR
5   GLYTHRGLYGLUARGVALLYSLEUPROGLY
6   METALAPHEASPLYSPROASNVALTYRGLU
7   PHEGLYTHRLYSTYRGLUASPILETYRARG
8   ASPLEUGLUSERLYSASPLYSGLUPHETYR
9   THRGLNASNGLYLEULEUHISMETLEUASP
10   ARGASNARGARGILELYSLYSCYSPROGLU
11   ARGPHEGLNASPTHRLYSGLUGLNPHEASP
12   ILEILEVALTHRVALGLUGLUARGVALTYR
13   ASPLEUVALVALMETHISMETGLUSERMET
14   GLUSERVALASPASNARGPROVALHISVAL
15   LEUASNVALASPVALVALASPASNALAGLU
16   ASPALALEUMETGLYALAPHEVALILETHR
17   ASPMETILEASNMETMETALALYSSERTHR
18   ASPLEUASPASNASPILEASPGLULEUILE
19   GLNGLUPHEGLUGLUARGARGLYSARGVAL
20   ILELEUHISSERVALLEUPHETYR

Entity 2, inorganic phosphate - O4 P - 94.971 Da.

1   PO4

Samples:

sample_2: Ssu72, [U-100% 13C; U-100% 15N], 1 mM; H2O 95%; D2O 5%; Sodium phosphate 25 mM; Potassium chloride 25 mM; dithiothreitol 2 mM

sample_3: Ssu72, [U-100% 13C; U-100% 15N], 1 mM; D2O 100%; Sodium phosphate 25 mM; Potassium chloride 25 mM; dithiothreitol 2 mM

sample_4: Ssu72, [U-10% 13C], 1 mM; H2O 95%; D2O 5%; Sodium phosphate 25 mM; Potassium chloride 25 mM; dithiothreitol 2 mM

sample_1: Ssu72, [U-100% 13C; U-100% 15N; U-80% 2H], 1 mM; H2O 95%; D2O 5%; Sodium phosphate 25 mM; Potassium chloride 25 mM; dithiothreitol 2 mM

sample_conditions_1: ionic strength: 75 mM; pH: 8; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D HN(CA)HAsample_2isotropicsample_conditions_1
3D HN(COCA)HAsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_3isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1
3D 1H-13C NOESYsample_3isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_4isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks