BMRB Entry 17487

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17487

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for ICL3 of 5TH1A receptor
Deposition date:
2011-02-24
Original release date:
2011-02-24
Authors:
Kim, Young Mee; Gayen, shovanlal; Chen, Angela; Huang, Qiwei; kang, congbao
Citation:

Citation: Chen, Angela Shuyi; Kim, Young Mee; Gayen, Shovanlal; Huang, Qiwei; Raida, Manfred; Kang, Congbao. "NMR structural study of the intracellular loop 3 of the serotonin 5-HT(1A) receptor and its interaction with calmodulin."  Biochim. Biophys. Acta 1808, 2224-2232 (2011).
PubMed: 21640070

Assembly members:

Assembly members:
ICL3_of_5-HT1A_receptor, polymer, 147 residues, 16043.0 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ICL3_of_5-HT1A_receptor: MGHHHHHHHHHHSSGHIEGR HMAARFRIRKTVKKVEKTGA DTRHGASPAPQPKKSVNGES GSRNWRLGVESKAGGALCAN GAVRQGDDGAALEVIEVHRV GNSKEHLPLPSEAGPTPCAP ASFERKNERNAEAKRKMALA RERKTVK

Data sets:
Data typeCount
13C chemical shifts351
15N chemical shifts111
1H chemical shifts211

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ICL3 of 5HT1A receptor1

Entities:

Entity 1, ICL3 of 5HT1A receptor 147 residues - 16043.0 Da.

1   METGLYHISHISHISHISHISHISHISHIS
2   HISHISSERSERGLYHISILEGLUGLYARG
3   HISMETALAALAARGPHEARGILEARGLYS
4   THRVALLYSLYSVALGLULYSTHRGLYALA
5   ASPTHRARGHISGLYALASERPROALAPRO
6   GLNPROLYSLYSSERVALASNGLYGLUSER
7   GLYSERARGASNTRPARGLEUGLYVALGLU
8   SERLYSALAGLYGLYALALEUCYSALAASN
9   GLYALAVALARGGLNGLYASPASPGLYALA
10   ALALEUGLUVALILEGLUVALHISARGVAL
11   GLYASNSERLYSGLUHISLEUPROLEUPRO
12   SERGLUALAGLYPROTHRPROCYSALAPRO
13   ALASERPHEGLUARGLYSASNGLUARGASN
14   ALAGLUALALYSARGLYSMETALALEUALA
15   ARGGLUARGLYSTHRVALLYS

Samples:

sample_1: ICL3 of 5-HT1A, [U-99% 13C; U-99% 15N], 0.5 mM; D2O, [U-99% 2H], 10%; sodium phosphate 20 mM; sodium chloride 150 mM; EDTA 2 mM; H2O 90%; DTT 1 mM

sample_2: ICL3 of 5-HT1A, [U-100% 15N], 0.5 mM; D2O, [U-99% 2H], 10%; sodium phosphate 20 mM; sodium chloride 150 mM; DTT 1 mM; EDTA 2 mM; H2O 90%

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1

Software:

NMRPipe, Bruker Biospin, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard - chemical shift assignment, collection, processing

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

GB AAD13945 AAL96692

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks