BMRB Entry 17483

Title:
Structural characterization of small heat shock protein (Hsp12) in aqueous solution
Deposition date:
2011-02-22
Original release date:
2011-10-26
Authors:
Singarapu, Kiran; Tonelli, Marco; Westler, William
Citation:

Citation: Singarapu, Kiran; Tonelli, Marco; Chow, Darius; Frederick, Ronnie; Westler, William; Markley, John. "Structural characterization of Hsp12, the heat shock protein from Saccharomyces cerevisiae, in aqueous solution where it is intrinsically disordered and in detergent micelles where it is locally -helical."  J. Biol. Chem. 286, 43447-43453 (2011).
PubMed: 21998307

Assembly members:

Assembly members:
hsp12, polymer, 109 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pvp68k

Data sets:
Data typeCount
13C chemical shifts293
15N chemical shifts99
1H chemical shifts387

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hsp121

Entities:

Entity 1, hsp12 109 residues - Formula weight is not available

1   METSERASPALAGLYARGLYSGLYPHEGLY
2   GLULYSALASERGLUALALEULYSPROASP
3   SERGLNLYSSERTYRALAGLUGLNGLYLYS
4   GLUTYRILETHRASPLYSALAASPLYSVAL
5   ALAGLYLYSVALGLNPROGLUASPASNLYS
6   GLYVALPHEGLNGLYVALHISASPSERALA
7   GLULYSGLYLYSASPASNALAGLUGLYGLN
8   GLYGLUSERLEUALAASPGLNALAARGASP
9   TYRMETGLYALAALALYSSERLYSLEUASN
10   ASPALAVALGLUTYRVALSERGLYARGVAL
11   HISGLYGLUGLUASPPROTHRLYSLYS

Samples:

sample_1: hsp12, [U-100% 15N], 0.2 mM; hsp12, [U-100% 13C; U-100% 15N], 1.0 mM

sample_conditions_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - data analysis

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Varian INOVA 900 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 17482 17948 18523
PDB
DBJ BAA09224 BAA14033 GAA23069
EMBL CAA39306 CAA86349 CAY79436
GB AAA34647 AAL06077 AAS56790 AHY75775 AJP38487
REF NP_116640
SP P22943
TPG DAA12425
AlphaFold P22943

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks