BMRB Entry 17347

Title:
Solution NMR structure of PAP248-286 in 30% TFE
Deposition date:
2010-12-03
Original release date:
2011-01-27
Authors:
NANGA, RAVI PRAKASH REDDY; BRENDER, JEFFREY; POPOVYCH, NATALIYA; RAMAMOORTHY, AYYALUSAMY
Citation:

Citation: Brender, Jeffrey; Nanga, Ravi Prakash Reddy; Popovych, Nataliya; Soong, Ronald; Macdonald, Peter; Ramamoorthy, Ayyalusamy. "The amyloidogenic SEVI precursor, PAP248-286, is highly unfolded in solution despite an underlying helical tendency."  Biochim. Biophys. Acta 1808, 1161-1169 (2011).
PubMed: 21262195

Assembly members:

Assembly members:
entity, polymer, 39 residues, 4561.504 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: obtained from a vendor

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GIHKQKEKSRLQGGVLVNEI LNHMKRATQIPSYKKLIMY

Data sets:
Data typeCount
1H chemical shifts269

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PAP248-2861

Entities:

Entity 1, PAP248-286 39 residues - 4561.504 Da.

1   GLYILEHISLYSGLNLYSGLULYSSERARG
2   LEUGLNGLYGLYVALLEUVALASNGLUILE
3   LEUASNHISMETLYSARGALATHRGLNILE
4   PROSERTYRLYSLYSLEUILEMETTYR

Samples:

sample_1: PAP248-286 1.2 mM; sodium phosphate 20 mM; D2O, [U-99% 2H], 10%; TFE, [U-99% 2H], 30%

sample_conditions_1: ionic strength: 0 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

TALOS, Cornilescu, Delaglio and Bax - refinement

Molmol, Koradi, Billeter and Wuthrich - structure solution

SPARKY, Goddard - chemical shift assignment, data analysis

ProcheckNMR, Laskowski and MacArthur - data analysis, structure solution

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker Avance 900 MHz

Related Database Links:

BMRB 17193 17346 17924 17925 18287
PDB
DBJ BAD89417 BAG62248
EMBL CAA36422 CAA37673
GB AAA60021 AAA60022 AAA69694 AAB60640 AAH07460
REF NP_001090 NP_001127666 NP_001278966 XP_001115549 XP_001148736
SP P15309
AlphaFold P15309