BMRB Entry 17320

Title:
NMR structure of the protein YP_926445.1 from Shewanella Amazonensis
Deposition date:
2010-11-23
Original release date:
2012-07-25
Authors:
Serrano, Pedro; Geralt, Michael; Mohanty, Biswaranjan; Horst, Reto; Wuthrich, Kurt
Citation:

Citation: Serrano, Pedro; Geralt, Michael; Mohanty, Biswaranjan; Horst, Reto; Wuthrich, Kurt. "NMR structure of the protein YP_926445.1 from Shewanella Amazonensis"  .

Assembly members:

Assembly members:
YP_926445.1, polymer, 114 residues, 12793.651 Da.

Natural source:

Natural source:   Common Name: Shewanella amazonensis   Taxonomy ID: 60478   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Shewanella amazonensis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSpeedET

Data sets:
Data typeCount
13C chemical shifts385
15N chemical shifts119
1H chemical shifts785

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YP_926445.11

Entities:

Entity 1, YP_926445.1 114 residues - 12793.651 Da.

1   METGLYALAGLYGLNTHRPROHISPROGLN
2   LEUILETRPPROALALEULEULYSGLNGLN
3   GLYCYSASNGLULEULEUPROLEUARGTHR
4   ASNASPASPTRPGLNARGPHECYSALAASP
5   SERLYSHISLEULEUGLNTYRGLYASPLYS
6   LEUVALASPSERASNPHEHISCYSPHEVAL
7   LEUGLUGLUASPALAHISTRPHISPROALA
8   ALAPROLEUPROPROGLUGLYLEUASNASP
9   LEUILEARGALAHISCYSALATHRLEUGLY
10   HISCYSCYSTHRSERLYSMETHISLEUHIS
11   SERVALMETASPALAILEASPPHELEUASN
12   ALALEUGLUGLY

Samples:

sample_1: sodium phosphate 20 mM; sodium chloride 50 mM; DTT 8 mM; sodium azide 4.5 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.113 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY (ali)sample_1isotropicsample_conditions_1
3D 1H-15N NOESY (Aro)sample_1isotropicsample_conditions_1
5D APSY-HACACONHsample_1isotropicsample_conditions_1
4D APSY-HACANHsample_1isotropicsample_conditions_1
5D APSY-CBCACONHsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

OPALp, Luginbuhl, Guntert, Billeter and Wuthrich - refinement

TOPSPIN v2.1, Bruker Biospin - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking

UNIO, Herrmann and Wuthrich - chemical shift assignment, peak picking, structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB ABL98775
REF WP_011758685

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks