BMRB Entry 17252

Title:
Solution NMR Structure of BCMA
Deposition date:
2010-10-12
Original release date:
2013-06-04
Authors:
Pellegrini, M.; Willen, L.; Perroud, M.; Krushinskie, D.; Strauch, K.; Cuervo, H.; Sun, Y.; Day, E.; Schneider, P.; Zheng, T.
Citation:

Citation: Pellegrini, Maria; Willen, Laure; Perroud, Mai; Krushinskie, Dennis; Strauch, Kathy; Cuervo, Hernan; Day, Eric; Schneider, Pascal; Zheng, Timothy. "Structure of the extracellular domains of human and Xenopus Fn14: implications in the evolution of TWEAK and Fn14 interactions."  FEBS J. 280, 1818-1829 (2013).
PubMed: 23438059

Assembly members:

Assembly members:
BCMA, polymer, 50 residues, 5532.333 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis   Host organism: N/A   Vector: N/A

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts182

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BCMA1

Entities:

Entity 1, BCMA 50 residues - 5532.333 Da.

an additional Cys (acetamidomethyl protected) was added to the C-terminus of the sythetic construct

1   LEUGLNMETALAGLYGLNCYSSERGLNASN
2   GLUTYRPHEASPSERLEULEUHISALACYS
3   ILEPROCYSGLNLEUARGCYSSERSERASN
4   THRPROPROLEUTHRCYSGLNARGTYRCYS
5   ASNALASERVALTHRASNSERVALLYSCY1

Samples:

sample: BCMA 900 uM; sodium phosphate 10 mM; sodium azide 0.02%; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 10 mM; pH: 6.0; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYsampleisotropicsample_conditions_1

Software:

CNS v1.1, Brunger A. T. et.al. - structure solution

SPARKY v3.11, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz MHz

Related Database Links:

PDB
DBJ BAB60895 BAI47121
EMBL CAA78679 CAA78680 CAA82690 CAA82691
GB AAB67251 AAH58291 AAR84240 AAV92616 AAX36273
REF NP_001183 XP_002826185 XP_003269345 XP_003832741 XP_004057277
SP Q02223
AlphaFold Q02223