BMRB Entry 17229

Title:
C-terminal zinc knuckle of the HIVNCp7 with DNA
Deposition date:
2010-10-01
Original release date:
2012-03-09
Authors:
Quintal, Susana; Viegas, Aldino; Cabrita, Eurico; Farrell, Nicholas; Erhardt, Stefan
Citation:

Citation: Quintal, Susana; Viegas, Aldino; Erhardt, Stefan; Cabrita, Eurico; Farrell, Nicholas. "Platinated DNA Affects Zinc Finger Conformation. Interaction of a Platinated Single-Stranded Oligonucleotide and the C-Terminal Zinc Finger of Nucleocapsid Protein HIVNCp7."  Biochemistry 51, 1752-1761 (2012).
PubMed: 22303928

Assembly members:

Assembly members:
C-terminal_zinc_knuckle_of_the_HIVNCp7, polymer, 19 residues, 2230.615 Da.
DNA, polymer, 6 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: HIV   Taxonomy ID: 12721   Superkingdom: viruses   Kingdom: not available   Genus/species: Lentivirus HIV

Experimental source:

Experimental source:   Production method: obtained from a vendor

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C-terminal_zinc_knuckle_of_the_HIVNCp7: KGCWKCGKEGHQMKDCTER
DNA: TACGCC

Data sets:
Data typeCount
1H chemical shifts153

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22
3ZINC_ION_13
4ZINC_ION_23
5ZINC_ION_33
6ZINC_ION_43

Entities:

Entity 1, entity_1 19 residues - 2230.615 Da.

1   LYSGLYCYSTRPLYSCYSGLYLYSGLUGLY
2   HISGLNMETLYSASPCYSTHRGLUARG

Entity 2, entity_2 6 residues - Formula weight is not available

1   DTDADCDGDCDC

Entity 3, ZINC_ION_1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: C-terminal zinc knuckle of the HIVNCp7 1 mM; DNA 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 6 mM; pH: 5.7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

SPARKY v3.114, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 1656 17216 17228 18980 2217 25532
PDB
DBJ BAA12988 BAA12996 BAA84661 BAA84662 BAA84669
EMBL CAA28011 CAA42971 CAA42973 CAA73647 CAA77483
GB AAA43911 AAA44201 AAA44313 AAA44314 AAA44317
PIR FOVWLV
PRF 1102247B 1103299C
REF NP_057849 NP_057850 NP_579881
SP O89939 O89940 P03347 P03348 P03366
AlphaFold O89940 P03347 P03348 P03366 O89939