BMRB Entry 17195

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17195

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Title:
Backbone assignments for the UBA Domain of E2-25K
Deposition date:
2010-09-15
Original release date:
2011-03-24
Authors:
Wilson, Randall; Edmondson, Stephen; Flatt, Justin; Twigg, Pamela
Citation:

Citation: Wilson, Randall; Edmondson, Stephen; Flatt, Justin; Helms, Kimberli; Twigg, Pamela. "The E2-25K ubiquitin-associated (UBA) domain aids in polyubiquitin chain synthesis and linkage specificity"  Biochem. Biophys. Res. Commun. 405, 662-666 (2011).
PubMed: 21281599

Assembly members:

Assembly members:
E2-25K_UBA_domain, polymer, 70 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
E2-25K_UBA_domain: MGSSHHHHHHSSGLVPEGSH MASGAPVSSPEYTKKIENLC AMGFDRNAVIVALSSKSWDV ETATELLLSN

Data sets:
Data typeCount
13C chemical shifts155
15N chemical shifts50
1H chemical shifts50
residual dipolar couplings37

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UBA monomer1

Entities:

Entity 1, UBA monomer 70 residues - Formula weight is not available

Residues 1-23 represent a non-native affinity tag and residues 24-70 represent residues 154-200 of E2-25K

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROGLUGLYSERHIS
3   METALASERGLYALAPROVALSERSERPRO
4   GLUTYRTHRLYSLYSILEGLUASNLEUCYS
5   ALAMETGLYPHEASPARGASNALAVALILE
6   VALALALEUSERSERLYSSERTRPASPVAL
7   GLUTHRALATHRGLULEULEULEUSERASN

Samples:

sample_1: E2-25K UBA domain, [U-100% 13C; U-100% 15N], 1.4 mM; DSS 0.12 mM; sodium azide 0.9 % v/v; sodium phosphate 50 mM; sodium chloride 100 mM; beta-mercaptoethanol 0.1 mM; H2O 90%; D2O 10%

RDC_sample: E2-25K UBA domain, [U-100% 15N], 0.98 mM; DSS 0.12 mM; sodium phosphate 50 mM; sodium chloride 100 mM; beta-mercaptoethanol 0.1 mM; n-dodecyl hexa(ethylene glycol) 5%; n-hexanol 5%; H2O 90%; D2O 10%

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 273 K

RDC_conditions: pH: 7; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
2D Nhsqc-ipapRDC_sampleisotropicRDC_conditions
2D NHSC S3RDC_sampleisotropicRDC_conditions

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

VNMRJ, Varian - collection

Module, Dosset, P., Hus, J. C., Marion, D., and Blackledge, M - data analysis

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks