BMRB Entry 16680

Title:
Solution structure of human stem cell transcription factor Nanog
Deposition date:
2010-01-15
Original release date:
2012-08-03
Authors:
Sahu, S.; Markley, J.
Citation:

Citation: Sahu, S.; Markley, J.. "Solution structure of human stem cell transcription factor Nanog"  To be published ., .-..

Assembly members:

Assembly members:
Nanog, polymer, 84 residues, 10068.668 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pVP68K

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts396
15N chemical shifts81
1H chemical shifts651

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Nanog1

Entities:

Entity 1, Nanog 84 residues - 10068.668 Da.

1   SERLYSGLNPROTHRSERALAGLUASNSER
2   VALALALYSLYSGLUASPLYSVALPROVAL
3   LYSLYSGLNLYSTHRARGTHRVALPHESER
4   SERTHRGLNLEUCYSVALLEUASNASPARG
5   PHEGLNARGGLNLYSTYRLEUSERLEUGLN
6   GLNMETGLNGLULEUSERASNILELEUASN
7   LEUSERTYRLYSGLNVALLYSTHRTRPPHE
8   GLNASNGLNARGMETLYSSERLYSARGTRP
9   GLNLYSASNASN

Samples:

sample_1: Nanog, [U-95% 13C; U-90% 15N], 0.7 mM; MOPS 10 mM; Dithiothreitol 5 mM; NaCl 250 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 250 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D_15N-separated_NOESYsample_1isotropicsample_conditions_1
3D_13C-separated_NOESYsample_1isotropicsample_conditions_1
3D_13C-separated_NOESY (AROMATIC)sample_1isotropicsample_conditions_1

Software:

CNS v1.21, Brunger A. T. et.al. - refinement

NMR spectrometers:

  • Varian Inova 600 MHz

Related Database Links:

PDB
DBJ BAB14151 BAC76999 BAF83585
GB AAI60187 AAP49529 AAT81526 ABA27089 ABZ92376
REF NP_001065295 NP_001284627 NP_079141 XP_003811843 XP_011519152
SP Q3LTE0 Q6NSW7 Q9H9S0
AlphaFold Q6NSW7 Q9H9S0 Q3LTE0

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks