BMRB Entry 16617

Title:
Solution structure of Avian Thymic Hormone
Deposition date:
2009-11-24
Original release date:
2010-03-22
Authors:
Henzl, Michael
Citation:

Citation: Schuermann, Jonathan; Tan, Anmin; Tanner, John; Henzl, Michael. "Structure of avian thymic hormone, a high-affinity avian beta-parvalbumin, in the Ca2+-free and Ca2+-bound states."  J. Mol. Biol. 397, 991-1002 (2010).
PubMed: 20156445

Assembly members:

Assembly members:
ATH, polymer, 108 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11

Data sets:
Data typeCount
13C chemical shifts428
15N chemical shifts110
1H chemical shifts729

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ATH1

Entities:

Entity 1, ATH 108 residues - Formula weight is not available

1   ALAILETHRASPILELEUSERALALYSASP
2   ILEGLUSERALALEUSERSERCYSGLNALA
3   ALAASPSERPHEASNTYRLYSSERPHEPHE
4   SERTHRVALGLYLEUSERSERLYSTHRPRO
5   ASPGLNILELYSLYSVALPHEGLYILELEU
6   ASPGLNASPLYSSERGLYPHEILEGLUGLU
7   GLUGLULEUGLNLEUPHELEULYSASNPHE
8   SERSERSERALAARGVALLEUTHRSERALA
9   GLUTHRLYSALAPHELEUALAALAGLYASP
10   THRASPGLYASPGLYLYSILEGLYVALGLU
11   GLUPHEGLNSERLEUVALLYSALA

Samples:

sample_1: sodium chloride 150 ± 0.3 mM; MES 10 ± 0.2 mM; THP 5 ± 0.2 mM; ATH, [U-98% 13C; U-98% 15N], 3 ± 0.3 mM

sample_2: sodium chloride 150 ± 0.3 mM; MES 10 ± 0.2 mM; THP 5 ± 0.2 mM; ATH, [U-15% 13C; U-98% 15N], 3 ± 0.3 mM

sample_3: sodium chloride 150 ± 0.3 mM; MES 10 ± 0.2 mM; THP 5 ± 0.2 mM; ATH, [U-98% 15N], 3 ± 0.3 mM

sample_conditions_1: ionic strength: 0.16 M; pH: 6.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCACOCANHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
2D HBCBCGCDHDsample_1isotropicsample_conditions_1
2D HBCBCGCDCEHEsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment, peak picking

VNMRJ, Varian - collection

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB AAA48605 AAA48606 EMC89994 EOB00978 KFM12340
REF NP_001007478 XP_003210593 XP_005020104 XP_005229015 XP_005442564
SP P19753
AlphaFold P19753

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks