BMRB Entry 16328

Title:
the S173N variant of rabbit prion protein (91-228)
Deposition date:
2009-05-30
Original release date:
2010-07-20
Authors:
Li, Jun; Wen, Yi; Lin, Donghai
Citation:

Citation: Wen, Yi; Li, Jun; Yao, Wenming; Xiong, Minqian; Hong, Jing; Peng, Yu; Xiao, Gengfu; Lin, Donghai. "Unique structural characteristics of the rabbit prion protein."  J. Biol. Chem. 285, 31682-31693 (2010).
PubMed: 20639199

Assembly members:

Assembly members:
prion_protein, polymer, 145 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Rabbit   Taxonomy ID: 9986   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Oryctolagus cuniculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET30a

Data sets:
Data typeCount
13C chemical shifts315
15N chemical shifts126
1H chemical shifts714

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1prion protein1

Entities:

Entity 1, prion protein 145 residues - Formula weight is not available

1   METGLNGLYGLYTHRHISASNGLNTRPGLY
2   LYSPROSERLYSPROLYSTHRSERMETLYS
3   HISVALALAGLYALAALAALAALAGLYALA
4   VALVALGLYGLYLEUGLYGLYTYRMETLEU
5   GLYSERALAMETSERARGPROLEUILEHIS
6   PHEGLYASNASPTYRGLUASPARGTYRTYR
7   ARGGLUASNMETTYRARGTYRPROASNGLN
8   VALTYRTYRARGPROVALASPGLNTYRSER
9   ASNGLNASNASNPHEVALHISASPCYSVAL
10   ASNILETHRVALLYSGLNHISTHRVALTHR
11   THRTHRTHRLYSGLYGLUASNPHETHRGLU
12   THRASPILELYSILEMETGLUARGVALVAL
13   GLUGLNMETCYSILETHRGLNTYRGLNGLN
14   GLUSERGLNALAALATYRGLNARGALAHIS
15   HISHISHISHISHIS

Samples:

sample: prion_protein, [U-100% 13C; U-100% 15N], 1 mM; sodium acetate 20 mM; H2O 90%; D2O 10%

sample_condition: ionic strength: 0.04 M; pH: 4.5; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
standard NMR experimentssampleisotropicsample_condition

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 15394 15399 16616
PDB
EMBL CBK44083
GB AAC48697 AAD01554 ABL75505 AEM44421 AEM44422
REF NP_001075490 XP_008254357 XP_008254358
SP Q95211
AlphaFold Q95211

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks