BMRB Entry 15935

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15935

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Pfu RPP29d17-RPP21V14 complex
Deposition date:
2008-08-30
Original release date:
2015-07-23
Authors:
Xu, Yiren; Amero, Carlos; Chen, Wen-Yi; Cho, I-Ming; Foster, Mark; Gopalan, Venkat
Citation:

Citation: Xu, Yiren; Amero, Carlos; Chen, Wen-Yi; Cho, I-Ming; Gopalan, Venkat; Foster, Mark. "Pfu RPP29d17-RPP21V14 complex"  .

Assembly members:

Assembly members:
Pfu_Rpp29, polymer, 111 residues, 28159.4 Da.
Pfu_Rpp21, polymer, 122 residues, 28159.4 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Pyrococcus furiosus   Taxonomy ID: 186497   Superkingdom: Archaea   Kingdom: not available   Genus/species: Pyrococcus furiosus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-33b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Pfu_Rpp29: MGSYQEIIGRTWIFRGAHRG RVNKKNIVWHELIGLKVRVV NSTHPGYVGIEGYVIDETRN MLVIAGENKVWKVPKDVCIF EFETWDGTKIKISGEKLVGR PEMRLKKRWRK
Pfu_Rpp21: AKYNEKKEKKRIVKERIDIL FSLAERVFPYSPELAKRYVE LALLVQQKAKVKIPRKWKRR YCKKCHAFLVPGINARVRLR QKRMPHIVVKCLECGHIMRY PYIKEIKKRRKEKMEYGGLV PR

Data sets:
Data typeCount
13C chemical shifts838
15N chemical shifts171
1H chemical shifts1317

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pfu Rpp291
2Pfu Rpp212
3ZN3

Entities:

Entity 1, Pfu Rpp29 111 residues - 28159.4 Da.

Residues 1-127 are from Pfu Rpp29. Residues 301-422 are from Pfu Rpp21

1   METGLYSERTYRGLNGLUILEILEGLYARG
2   THRTRPILEPHEARGGLYALAHISARGGLY
3   ARGVALASNLYSLYSASNILEVALTRPHIS
4   GLULEUILEGLYLEULYSVALARGVALVAL
5   ASNSERTHRHISPROGLYTYRVALGLYILE
6   GLUGLYTYRVALILEASPGLUTHRARGASN
7   METLEUVALILEALAGLYGLUASNLYSVAL
8   TRPLYSVALPROLYSASPVALCYSILEPHE
9   GLUPHEGLUTHRTRPASPGLYTHRLYSILE
10   LYSILESERGLYGLULYSLEUVALGLYARG
11   PROGLUMETARGLEULYSLYSARGTRPARG
12   LYS

Entity 2, Pfu Rpp21 122 residues - 28159.4 Da.

Residues 1-127 are from Pfu Rpp29. Residues 301-422 are from Pfu Rpp21

1   ALALYSTYRASNGLULYSLYSGLULYSLYS
2   ARGILEVALLYSGLUARGILEASPILELEU
3   PHESERLEUALAGLUARGVALPHEPROTYR
4   SERPROGLULEUALALYSARGTYRVALGLU
5   LEUALALEULEUVALGLNGLNLYSALALYS
6   VALLYSILEPROARGLYSTRPLYSARGARG
7   TYRCYSLYSLYSCYSHISALAPHELEUVAL
8   PROGLYILEASNALAARGVALARGLEUARG
9   GLNLYSARGMETPROHISILEVALVALLYS
10   CYSLEUGLUCYSGLYHISILEMETARGTYR
11   PROTYRILELYSGLUILELYSLYSARGARG
12   LYSGLULYSMETGLUTYRGLYGLYLEUVAL
13   PROARG

Entity 3, ZN - Zn - 65.409 Da.

1   ZN

Samples:

15N_13C-Rpp29_unlabeled_Rpp21: Pfu Rpp29, [U-95% 13C; U-95% 15N], 1-2 mM; Pfu Rpp21 1-2 mM; TRIS 10 mM; potassium chloride 10 mM; ZN 0.3 mM; sodium azide 0.02%; D2O, [U-2H], 10%; H2O 90%

15N_13C-Rpp29_unlabeled_Rpp21_in_D2O: Pfu Rpp29, [U-95% 13C; U-95% 15N], 1-2 mM; Pfu Rpp21 1-2 mM; TRIS 10 mM; potassium chloride 10 mM; ZN 0.3 mM; sodium azide 0.02%; D2O, [U-2H], 100%

15N_13C-Rpp21_unlabeled_Rpp29: Pfu Rpp21, [U-95% 13C; U-95% 15N], 1-2 mM; Pfu Rpp29 1-2 mM; TRIS 10 mM; potassium chloride 10 mM; ZN 0.3 mM; sodium azide 0.02%; D2O, [U-2H], 10%; H2O 90%

15N_13C-Rpp21_unlabeled_Rpp29_in_D2O: Pfu Rpp21, [U-95% 13C; U-95% 15N], 1-2 mM; Pfu Rpp29 1-2 mM; TRIS 10 mM; potassium chloride 10 mM; ZN 0.3 mM; sodium azide 0.02%; D2O, [U-2H], 100%

ILV-methyl_1H_15N_13C_2H-Rpp29_unlabeled_Rpp21: Pfu Rpp29, [ILV methyl-1H, U-15N, U-13C, 2H], 1-2 mM; Pfu Rpp21 1-2 mM; TRIS 10 mM; potassium chloride 10 mM; ZN 0.3 mM; sodium azide 0.02%; D2O, [U-2H], 10%; H2O 90%

15N_13C-Rpp29: Pfu Rpp29, [U-95% 13C; U-95% 15N], 1-2 mM; TRIS 10 mM; potassium chloride 10 mM; ZN 0.3 mM; sodium azide 0.02%; D2O, [U-2H], 10%; H2O 90%

15N_13C-Rpp21: Pfu Rpp21, [U-95% 13C; U-95% 15N], 1-2 mM; TRIS 10 mM; potassium chloride 10 mM; ZN 0.3 mM; sodium azide 0.02%; D2O, [U-2H], 10%; H2O 90%

sample_conditions_H2O: ionic strength: 32.3 mM; pH: 6.7; pressure: 1 atm; temperature: 328 K

sample_conditions_D2O: ionic strength: 32.3 mM; pD: 7.1; pressure: 1 atm; temperature: 328 K

Experiments:

NameSampleSample stateSample conditions
3D HNCO15N_13C-Rpp29_unlabeled_Rpp21isotropicsample_conditions_H2O
3D HNCA15N_13C-Rpp29_unlabeled_Rpp21isotropicsample_conditions_H2O
3D HNCACB15N_13C-Rpp29_unlabeled_Rpp21isotropicsample_conditions_H2O
3D CBCA(CO)NH15N_13C-Rpp29_unlabeled_Rpp21isotropicsample_conditions_H2O
3D HBHA(CO)NH15N_13C-Rpp29_unlabeled_Rpp21isotropicsample_conditions_H2O
3D HCCH-COSY15N_13C-Rpp29_unlabeled_Rpp21isotropicsample_conditions_H2O
3D HCCH-TOCSY15N_13C-Rpp29_unlabeled_Rpp21isotropicsample_conditions_H2O
3D 1H-15N NOESY15N_13C-Rpp29_unlabeled_Rpp21isotropicsample_conditions_H2O
3D 1H-13C NOESY15N_13C-Rpp29_unlabeled_Rpp21_in_D2Oisotropicsample_conditions_D2O
3D Aromatic 1H-13C NOESY15N_13C-Rpp29_unlabeled_Rpp21_in_D2Oisotropicsample_conditions_D2O
3D 13C-filtered/edited NOESY15N_13C-Rpp29_unlabeled_Rpp21_in_D2Oisotropicsample_conditions_D2O
3D H(CCO)NHILV-methyl_1H_15N_13C_2H-Rpp29_unlabeled_Rpp21isotropicsample_conditions_H2O
3D C(CO)NHILV-methyl_1H_15N_13C_2H-Rpp29_unlabeled_Rpp21isotropicsample_conditions_H2O
3D 15N-edited NCH NOESYILV-methyl_1H_15N_13C_2H-Rpp29_unlabeled_Rpp21isotropicsample_conditions_H2O
3D 15N, 13C-edited HNNH, HCNH time shared NOESYILV-methyl_1H_15N_13C_2H-Rpp29_unlabeled_Rpp21isotropicsample_conditions_H2O
2D 1H-15N HSQC15N_13C-Rpp29isotropicsample_conditions_H2O
2D 1H-15N HSQC15N_13C-Rpp29_unlabeled_Rpp21isotropicsample_conditions_H2O
3D HNCO15N_13C-Rpp21_unlabeled_Rpp29isotropicsample_conditions_H2O
3D HNCA15N_13C-Rpp21_unlabeled_Rpp29isotropicsample_conditions_H2O
3D HNCACB15N_13C-Rpp21_unlabeled_Rpp29isotropicsample_conditions_H2O
3D CBCA(CO)NH15N_13C-Rpp21_unlabeled_Rpp29isotropicsample_conditions_H2O
3D H(CCO)NH15N_13C-Rpp21_unlabeled_Rpp29isotropicsample_conditions_H2O
3D C(CO)NH15N_13C-Rpp21_unlabeled_Rpp29isotropicsample_conditions_H2O
3D HCCH-COSY15N_13C-Rpp21_unlabeled_Rpp29_in_D2Oisotropicsample_conditions_D2O
3D Aromatic 1H-13C NOESY15N_13C-Rpp21_unlabeled_Rpp29_in_D2Oisotropicsample_conditions_D2O
3D 1H-15N NOESY15N_13C-Rpp21_unlabeled_Rpp29isotropicsample_conditions_H2O
3D 1H-13C NOESY15N_13C-Rpp21_unlabeled_Rpp29_in_D2Oisotropicsample_conditions_D2O
3D 13C-filtered/edited NOESY15N_13C-Rpp21_unlabeled_Rpp29isotropicsample_conditions_D2O

Software:

xwinnmr, Bruker Biospin - collection

CARA, Keller and Wuthrich - data analysis

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

TALOS, Cornilescu, Delaglio and Bax - data analysis

SANE, Duggan, Legge, Dyson & Wright - data analysis

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - geometry optimization, refinement, structure solution

ProcheckNMR, Laskowski and MacArthur - data analysis

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

BMRB 16266
PDB
GB AAL81940
REF WP_048059096
SP Q8U007
AlphaFold Q8U007

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks