BMRB Entry 15586
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15586
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Ellison, Michael; Feng, Zhi-Ping; Park, Anthony; Zhang, Xuecheng; McIntosh, Michael; Olivera, Baldomero; Norton, Raymond. "alpha-RgIA, a Novel Conotoxin That Blocks the alpha9alpha10 nAChR: Structure and
Identification of Key Receptor-Binding Residues." J. Mol. Biol. 377, 1216-1227 (2008).
PubMed: 18295795
Assembly members:
RgIA, polymer, 13 residues, Formula weight is not available
Natural source: Common Name: Conus regius Taxonomy ID: 101314 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Conus regius
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
RgIA: GCCSDPRCAYRCR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 74 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RgIA | 1 |
Entities:
Entity 1, RgIA 13 residues - Formula weight is not available
| 1 | GLY | CYS | CYS | SER | ASP | PRO | ARG | CYS | ALA | TYR | ||||
| 2 | ARG | CYS | ARG |
Samples:
sample_1: RgIA 0.6 mM; D2O, [U-100% 2H], 5%; H2O 95%
sample_conditions_1: ionic strength: 0 M; pH: 5.1; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - chemical shift assignment
NMR spectrometers:
- Bruker DRX 600 MHz