BMRB Entry 15561

Name: NMR resonance assignments for Ca2+-bound DREAM
Published: 2008-03-17

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15561

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR resonance assignments for Ca2+-bound DREAM

Deposition date:

2007-11-24

Original release date:

2008-03-17

Authors:

Ames, James

Citation:

Citation: Lusin, J.; Vanarotti, M.; Li, C.; Valiveti, A.; Ames, James. "NMR Structure of DREAM: Implications for Ca2+-dependent DNA binding and Protein Dimerization" Biochemistry 47, 2252-2264 (2008).
PubMed: 18201103

Assembly members:

Assembly members:
DREAM, polymer, 192 residues, Formula weight is not available
CA, non-polymer, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
DREAM: SELELSTVRHQPEGLDQLQA QTKFTKKELQSLYRGFKNEC PTGLVDEDTFKLIYSQFFPQ GDATTYAHFLFNAFDADGNG AIHFEDFVVGLSILLRGTVH EKLKWAFNLYDINKDGCITK EEMLAIMKSIYDMMGRHTYP ILREDAPLEHVERFFQKMDR NQDGVVTIDEFLETCQKDEN ITNSMQLFENVI

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	370
15N chemical shifts	183
1H chemical shifts	365

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	subunit 1	1
2	CALCIUM ION	2

Entities:

Entity 1, subunit 1 192 residues - Formula weight is not available

The first 64 residues of the full-length protein have been deleted. The first residue in this deletion construct is S65.

1

SER

GLU

LEU

GLU

LEU

SER

THR

VAL

ARG

HIS

2

GLN

PRO

GLU

GLY

LEU

ASP

GLN

LEU

GLN

ALA

3

GLN

THR

LYS

PHE

THR

LYS

GLU

LEU

GLN

4

SER

LEU

TYR

ARG

GLY

PHE

LYS

ASN

GLU

CYS

5

PRO

THR

GLY

LEU

VAL

ASP

GLU

ASP

THR

PHE

6

LYS

LEU

ILE

TYR

SER

GLN

PHE

PRO

GLN

7

GLY

ASP

ALA

THR

TYR

ALA

HIS

PHE

LEU

8

PHE

ASN

ALA

PHE

ASP

ALA

ASP

GLY

ASN

GLY

9

ALA

ILE

HIS

PHE

GLU

ASP

PHE

VAL

GLY

10

LEU

SER

ILE

LEU

ARG

GLY

THR

VAL

HIS

11

GLU

LYS

LEU

LYS

TRP

ALA

PHE

ASN

LEU

TYR

12

ASP

ILE

ASN

LYS

ASP

GLY

CYS

ILE

THR

LYS

13

GLU

MET

LEU

ALA

ILE

MET

LYS

SER

ILE

14

TYR

ASP

MET

GLY

ARG

HIS

THR

TYR

PRO

15

ILE

LEU

ARG

GLU

ASP

ALA

PRO

LEU

GLU

HIS

16

VAL

GLU

ARG

PHE

GLN

LYS

MET

ASP

ARG

17

ASN

GLN

ASP

GLY

VAL

THR

ILE

ASP

GLU

18

PHE

LEU

GLU

THR

CYS

GLN

LYS

ASP

GLU

ASN

19

ILE

THR

ASN

SER

MET

GLN

LEU

PHE

GLU

ASN

20

VAL

ILE

Entity 2, CALCIUM ION - Formula weight is not available

1

CA

Samples:

sample_1: DREAM, [U-98% 13C; U-98% 15N], 0.5 mM; CALCIUM ION 0.5 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 7.4; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker DRX 600 MHz

PDB	2JUL
DBJ	BAA96360 BAE38349
GB	AAF14576 AAF74784 AAG15382 AAG17450 AAH26980
REF	NP_001069080 NP_001104801 NP_001277934 NP_062763 NP_115851
SP	Q17QD9 Q9JM47 Q9QXT8
AlphaFold	Q9QXT8 Q9JM47 Q17QD9