BMRB Entry 15446

Title:
2cxxc, YgiT
Deposition date:
2007-08-28
Original release date:
2008-01-30
Authors:
Papadopoulos, Evangelos; Gardikas Vlamis, Alexios; Graslund, Astrid; Billeter, Martin
Citation:

Citation: Papadopoulos, Evangelos; Billeter, Martin; Graslund, Astrid; Vlamis-Gardikas, Alexios. "Assignment of (1)H, (13)C, and (15)N resonances of YgiT, a putative DNA interacting protein from E. coli, containing one HTH and two CxxC motifs"  Biomol. NMR Assignments 1, 217-219 (2007).
PubMed: 19636869

Assembly members:

Assembly members:
2cxxc_-_Ygit, polymer, 131 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: Escherichia coli

Data sets:
Data typeCount
13C chemical shifts510
15N chemical shifts127
1H chemical shifts685

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
12cxxc1
2Zinc ion2

Entities:

Entity 1, 2cxxc 131 residues - Formula weight is not available

1   METLYSCYSPROVALCYSHISGLNGLYGLU
2   METVALSERGLYILELYSASPILEPROTYR
3   THRPHEARGGLYARGLYSTHRVALLEULYS
4   GLYILEHISGLYLEUTYRCYSVALHISCYS
5   GLUGLUSERILEMETASNLYSGLUGLUSER
6   ASPALAPHEMETALAGLNVALLYSALAPHE
7   ARGALASERVALASNALAGLUTHRVALALA
8   PROGLUPHEILEVALLYSVALARGLYSLYS
9   LEUSERLEUTHRGLNLYSGLUALASERGLU
10   ILEPHEGLYGLYGLYVALASNALAPHESER
11   ARGTYRGLULYSGLYASNALAGLNPROHIS
12   PROSERTHRILELYSLEULEUARGVALLEU
13   ASPLYSHISPROGLULEULEUASNGLUILE
14   ARG

Entity 2, Zinc ion - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: 2cxxc - Ygit, [U-100% 13C; U-100% 15N], 1 mM; Zinc ion 1 mM; Sodium Phosphate buffer 20 mM

sample_conditions_1: ionic strength: 25 mM; pH: 5; pressure: 0.7 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

BMRB 17009
PDB
DBJ BAE77077 BAJ44773 BAL39680
EMBL CAQ33360 CAR14662 CAR19632 CBJ02792 CCJ45634
GB AAA69189 AAC76057 ABV07432 ACA76352 ACB04106
REF NP_417493 WP_000650107 WP_001487520 WP_021572968 WP_044814339
SP Q46864
AlphaFold Q46864

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks