BMRB Entry 15266

Title:
1H, 13C, and 15N Backbone Resonance Assignments of the Carboxyl Terminal Domain of Connexin40
Deposition date:
2007-05-24
Original release date:
2007-09-17
Authors:
Bouvier, Denis; Kieken, Fabien
Citation:

Citation: Bouvier, Denis; Kieken, Fabien; Sorgen, Paul. "(1)H, (13)C, and (15)N backbone resonance assignments of the carboxyl terminal domain of Connexin40"  Biomol. NMR Assignments 1, 155-157 (2007).
PubMed: 19636853

Assembly members:

Assembly members:
Connexin43, polymer, 109 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P-2

Data sets:
Data typeCount
13C chemical shifts418
15N chemical shifts98
1H chemical shifts692

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Connexin401

Entities:

Entity 1, Connexin40 109 residues - Formula weight is not available

1   GLYPROLEUGLYSERTHRSERLEUVALGLN
2   GLYLEUTHRPROPROPROASPPHEASNGLN
3   CYSLEULYSASNSERPROASPGLULYSPHE
4   PHESERASPPHESERASNASNMETGLYSER
5   ARGLYSASNPROASPPROLEUALATHRGLU
6   GLUVALPROASNGLNGLUGLNILEPROGLU
7   GLUGLYPHEILEHISTHRGLNTYRGLYGLN
8   LYSPROGLUGLNPROSERGLYALASERALA
9   GLYHISARGPHEPROGLNGLYTYRHISSER
10   ASPLYSARGARGLEUSERLYSALASERSER
11   LYSALAARGSERASPASPLEUSERVAL

Samples:

sample_1: Connexin40, [U-98% 13C; U-98% 15N], 1 mM; Phosphate 11.9 mM; Sodium Chloride 137 mM; Potassium Chloride 2.7 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.0; pressure: 1 atm; temperature: 280 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB AAA41000 AAA41194 AAC53502 AAC53503 AAH70935
REF NP_062153 XP_008759550
SP P28234
AlphaFold P28234

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks