BMRB Entry 12014

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for cyclophilin 1 from Trichomonas vaginalis
Deposition date:
2017-11-22
Original release date:
2018-12-11
Authors:
Martin, Tesmine; Wei, Shu-Yi; Chen, Chinpan
Citation:

Citation: Martin, Tesmine; Lou, Yuan-Chao; Chou, Chun-Chi; Wei, Shu-Yi; Sadotra, Sushant; Cho, Chao-Cheng; Lin, Meng-Hsuan; Tai, Jung-Hsiang; Hsu, Chun-Hua; Chen, Chinpan. "Structural basis of interaction between dimeric cyclophilin 1 and Myb1 transcription factor in Trichomonas vaginalis."  Sci. Rep. 8, 5410-5410 (2018).
PubMed: 29615721

Assembly members:

Assembly members:
TvCyP1, polymer, 175 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Trichomonas vaginalis   Taxonomy ID: 5722   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Trichomonas vaginalis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET 28a

Data sets:
Data typeCount
13C chemical shifts289
15N chemical shifts160
1H chemical shifts160

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1protomer 11
2protomer 21

Entities:

Entity 1, protomer 1 175 residues - Formula weight is not available

1   GLYSERMETLEULYSARGPROLYSTHRPHE
2   PHEASPILESERILEARGGLYASPLYSVAL
3   GLYLYSILEVALPHEGLULEUPHEASNASP
4   ILEVALPROLYSTHRALAGLUASNPHEARG
5   ALALEUCYSTHRGLYGLULYSGLYILEGLY
6   LYSSERGLYMETPROLEUSERTYRLYSGLY
7   THRMETPHEHISARGILEILEPROGLNPHE
8   METILEGLNGLYGLYASPPHETHRARGPHE
9   ASNGLYTHRGLYGLYGLUSERILETYRGLY
10   METLYSPHEASPASPGLUASNPHELYSVAL
11   LYSHISASPLYSPROGLYLEULEUSERMET
12   ALAASNALAGLYPROASNTHRASNGLYSER
13   GLNPHEPHEILETHRTHRVALGLUTHRPRO
14   TRPLEUASPGLYHISHISCYSVALPHEGLY
15   GLNVALILEGLUGLYMETASPILEVALLYS
16   GLNILEGLUSERCYSGLYTHRGLUSERGLY
17   ARGPROARGALAMETCYSMETVALTHRASP
18   CYSGLYGLUMETLYS

Samples:

sample_1: TvCyP1, [U-13C; U-15N; U-2H], 0.5 mM; sodium chloride 50 mM; sodium azide 0.5 mM; sodium phosphate 20 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: pH: 6.0; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks