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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11523
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Kobayashi, Ayaho; Kanaba, Teppei; Satoh, Ryosuke; Fujiwara, Toshinobu; Ito, Yutaka; Sugiura, Reiko; Mishima, Masaki. "Structure of the second RRM domain of Nrd1, a fission yeast MAPK target RNA binding protein, and implication for its RNA recognition and regulation" Biochem. Biophys. Res. Commun. 437, 12-17 (2013).
PubMed: 23770370
Assembly members:
Nrd1_RRM2, polymer, 97 residues, 10793.440 Da.
Natural source: Common Name: fission yeast Taxonomy ID: 4896 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Schizosaccharomyces pombe
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-49b(+)
Entity Sequences (FASTA):
Nrd1_RRM2: NSASNSSVLLAVQQSGACRN
VFLGNLPNGITEDEIREDLE
PFGPIDQIKIVTERNIAFVH
FLNIAAAIKAVQELPLNPKW
SKRRIYYGRDRCAVGLK
Data type | Count |
13C chemical shifts | 410 |
15N chemical shifts | 93 |
1H chemical shifts | 652 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Nrd1_RRM2 | 1 |
Entity 1, Nrd1_RRM2 97 residues - 10793.440 Da.
1 | ASN | SER | ALA | SER | ASN | SER | SER | VAL | LEU | LEU | ||||
2 | ALA | VAL | GLN | GLN | SER | GLY | ALA | CYS | ARG | ASN | ||||
3 | VAL | PHE | LEU | GLY | ASN | LEU | PRO | ASN | GLY | ILE | ||||
4 | THR | GLU | ASP | GLU | ILE | ARG | GLU | ASP | LEU | GLU | ||||
5 | PRO | PHE | GLY | PRO | ILE | ASP | GLN | ILE | LYS | ILE | ||||
6 | VAL | THR | GLU | ARG | ASN | ILE | ALA | PHE | VAL | HIS | ||||
7 | PHE | LEU | ASN | ILE | ALA | ALA | ALA | ILE | LYS | ALA | ||||
8 | VAL | GLN | GLU | LEU | PRO | LEU | ASN | PRO | LYS | TRP | ||||
9 | SER | LYS | ARG | ARG | ILE | TYR | TYR | GLY | ARG | ASP | ||||
10 | ARG | CYS | ALA | VAL | GLY | LEU | LYS |
sample_1: potassium phosphate 50 mM; sodium chloride 100 mM; TCEP 1 mM; EDTA 0.1 mM; Nrd1 RRM2, [U-99% 13C; U-99% 15N], 1.0 mM; H2O 93%; D2O 7%
sample_2: potassium phosphate 50 mM; sodium chloride 100 mM; TCEP 1 mM; EDTA 0.1 mM; Nrd1 RRM2, [U-99% 15N], 1.2 mM; H2O 93%; D2O 7%
sample_3: potassium phosphate 50 mM; sodium chloride 100 mM; TCEP 1 mM; EDTA 0.1 mM; Nrd1 RRM2, [U-99% 13C; U-99% 15N], 1.0 mM; D2O 100%
sample_4: potassium phosphate 50 mM; sodium chloride 100 mM; TCEP 1 mM; EDTA 0.1 mM; Nrd1 RRM2, [U-10% 13C; U-99% 15N], 0.35 mM; H2O 93%; D2O 7%
sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 303 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
4D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
3D 1H-13C NOESY | sample_3 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_2 | isotropic | sample_conditions_1 |
3D HNHB | sample_2 | isotropic | sample_conditions_1 |
3D HA(CA)HB | sample_3 | isotropic | sample_conditions_1 |
2D 1H-13C CT-HSQC | sample_4 | isotropic | sample_conditions_1 |
CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
SPARKY, Goddard - chemical shift assignment
TALOS, Cornilescu, Delaglio and Bax - data analysis
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
TOPSPIN, Bruker Biospin - collection
PDB |
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