BMRB Entry 11492

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR11492

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Title:
Dihydrofolate Reductase from E.coli G67 deletion mutant
Deposition date:
2012-03-30
Original release date:
2012-08-06
Authors:
Nakashima, Atsushi; Wada, Yuji; Tate, Shin-ichi
Citation:

Citation: Nakashima, Atsushi; Wada, Yuji; Tate, Shin-ichi. "Backbone Assignment of Dihydrofolate Reductase from E.coli G67 deletion mutant"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
Dihydrofolate_Reductase_from_E._coli, polymer, 158 residues, 17943.3 Da.
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, non-polymer, 743.405 Da.
FOLIC ACID, non-polymer, 441.397 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Dihydrofolate_Reductase_from_E._coli: MISLIAALAVDRVIGMENAM PWNLPADLAWFKRNTLNKPV IMGRHTWESIGRPLPGRKNI ILSSQPTDDRVTWVKSVDEA IAACGDVPEIMVIGGGRVYE QFLPKAQKLYLTHIDAEVEG DTHFPDYEPDDWESVFSEFH DADAQNSHSYCFEILERR

Data sets:
Data typeCount
13C chemical shifts289
15N chemical shifts129
1H chemical shifts129

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1monomer1
2NAP2
3FOL3

Entities:

Entity 1, monomer 158 residues - 17943.3 Da.

1   METILESERLEUILEALAALALEUALAVAL
2   ASPARGVALILEGLYMETGLUASNALAMET
3   PROTRPASNLEUPROALAASPLEUALATRP
4   PHELYSARGASNTHRLEUASNLYSPROVAL
5   ILEMETGLYARGHISTHRTRPGLUSERILE
6   GLYARGPROLEUPROGLYARGLYSASNILE
7   ILELEUSERSERGLNPROTHRASPASPARG
8   VALTHRTRPVALLYSSERVALASPGLUALA
9   ILEALAALACYSGLYASPVALPROGLUILE
10   METVALILEGLYGLYGLYARGVALTYRGLU
11   GLNPHELEUPROLYSALAGLNLYSLEUTYR
12   LEUTHRHISILEASPALAGLUVALGLUGLY
13   ASPTHRHISPHEPROASPTYRGLUPROASP
14   ASPTRPGLUSERVALPHESERGLUPHEHIS
15   ASPALAASPALAGLNASNSERHISSERTYR
16   CYSPHEGLUILELEUGLUARGARG

Entity 2, NAP - C21 H28 N7 O17 P3 - 743.405 Da.

1   NAP

Entity 3, FOL - C19 H19 N7 O6 - 441.397 Da.

1   FOL

Samples:

sample_1: Dihydrofolate Reductase from E. coli, [U-100% 13C; U-100% 15N], 1 mM; potassium phosphate 50 mM; potassium chloride 100 mM; EDTA 1 mM; DTT 1 mM; NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE 6 mM; FOLIC ACID 10 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.8; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

Kujira, RIKEN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

BMRB 25019 4554
PDB
DBJ BAA05974 BAB33474 BAB96616 BAG75573 BAI23410
EMBL CAA28755 CAP74618 CAQ30568 CAQ87642 CAQ96940
GB AAA87976 AAC73159 AAG54351 AAN41711 AAN78554
REF NP_308078 NP_414590 NP_706004 WP_000378105 WP_000624372
SP P0ABQ4 P0ABQ5 P0ABQ6
AlphaFold P0ABQ6 P0ABQ4 P0ABQ5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks