BMRB Entry 11467

Title:
Family GH19 chitinase from Rye seeds
Deposition date:
2011-12-19
Original release date:
2012-03-06
Authors:
Okazaki, Yoko; Shinya, Shoko; Ohnuma, Takayuki; Fukamizo, Tamo
Citation:

Citation: Okazaki, Yoko; Shinya, Shoko; Ohnuma, Takayuki; Fukamizo, Tamo. "chitinoligosaccharide binding mode of Family GH19 chitinase from rye seeds"  Biochemistry ., .-..

Assembly members:

Assembly members:
RSC-c, polymer, 243 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: rye   Taxonomy ID: 4550   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Secale cereale

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-22b

Data sets:
Data typeCount
13C chemical shifts530
15N chemical shifts194
1H chemical shifts194

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Family GH19 chitinase1

Entities:

Entity 1, Family GH19 chitinase 243 residues - Formula weight is not available

1   SERVALSERSERILEILESERHISALAGLN
2   PHEASPARGMETLEULEUHISARGASNASP
3   GLYALACYSGLNALALYSGLYPHETYRTHR
4   TYRASPALAPHEVALALAALAALAASNALA
5   PHEPROGLYPHEGLYALATHRGLYSERTHR
6   ASPALAARGLYSARGASPVALALAALAPHE
7   LEUALAGLNTHRSERHISGLUTHRTHRGLY
8   GLYTRPALATHRALAPROASPGLYALAPHE
9   ALATRPGLYTYRCYSPHELYSGLNGLUARG
10   GLYALAALAALAASPTYRCYSTHRPROSER
11   ALAGLNTRPPROCYSALAPROGLYLYSARG
12   TYRTYRGLYARGGLYPROILEGLNLEUSER
13   HISASNTYRASNTYRGLYPROALAGLYARG
14   ALAILEGLYVALASPLEULEUARGASNPRO
15   ASPLEUVALALATHRASPPROTHRVALSER
16   PHELYSTHRALALEUTRPPHETRPMETTHR
17   ALAGLNALAPROLYSPROSERSERHISALA
18   VALILETHRGLYLYSTRPSERPROSERGLY
19   ALAASPARGALAALAGLYARGALAPROGLY
20   PHEGLYVALILETHRASNILEILEASNGLY
21   GLYLEUGLUCYSGLYHISGLYGLNASPSER
22   ARGVALALAASPARGILEGLYPHETYRLYS
23   ARGTYRCYSASPILELEUGLYVALGLYTYR
24   GLYASPASNLEUASPCYSTYRASNGLNARG
25   PROPHEALA

Samples:

sample_1: RSC-c, [U-99% 13C; U-99% 15N], 0.3 mM; sodium acetate 50 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 5.0; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

PDB
DBJ BAB18520
SP Q9FRV0
AlphaFold Q9FRV0

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks