BMRB Entry 11457

Title:
Solution structure of the second dsRBD from RNA helicase A
Deposition date:
2011-11-29
Original release date:
2012-08-29
Authors:
Nagata, Takashi; Muto, Yutaka; Tsuda, Kengo; Inoue, Makoto; Kigawa, Takanori; Terada, Takaho; Shirouzu, Mikako; Yokoyama, Shigeyuki
Citation:

Citation: Nagata, Takashi; Tsuda, Kengo; Kobayashi, Naohiro; Shirouzu, Mikako; Kigawa, Takanori; Guntert, Peter; Yokoyama, Shigeyuki; Muto, Yutaka. "Solution structures of the double-stranded RNA-binding domains from RNA helicase A"  Proteins 80, 1699-1706 (2012).
PubMed: 22454253

Assembly members:

Assembly members:
entity, polymer, 113 residues, 12507.041 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: E. coli - cell free   Vector: not applicable

Data sets:
Data typeCount
13C chemical shifts485
15N chemical shifts122
1H chemical shifts771

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1second dsRBD1

Entities:

Entity 1, second dsRBD 113 residues - 12507.041 Da.

1   GLYSERSERGLYSERSERGLYLEUGLUSER
2   GLUGLUVALASPLEUASNALAGLYLEUHIS
3   GLYASNTRPTHRLEUGLUASNALALYSALA
4   ARGLEUASNGLNTYRPHEGLNLYSGLULYS
5   ILEGLNGLYGLUTYRLYSTYRTHRGLNVAL
6   GLYPROASPHISASNARGSERPHEILEALA
7   GLUMETTHRILETYRILELYSGLNLEUGLY
8   ARGARGILEPHEALAARGGLUHISGLYSER
9   ASNLYSLYSLEUALAALAGLNSERCYSALA
10   LEUSERLEUVALARGGLNLEUTYRHISLEU
11   GLYVALILEGLUALATYRSERSERGLYPRO
12   SERSERGLY

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 1.16 mM; sodium phosphate 20 mM; sodium chloride 100 mM; DTT 1 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - refinement

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAB28848 BAE24846 BAE88384 BAH90798
EMBL CAA58036 CAA71668 CAH92036
GB AAB48855 AAC05725 AAH08773 AAH14246 AAH25245
PIR A47363
REF NP_001100654 NP_001253117 NP_001348 NP_031868 NP_776461
SP O70133 Q08211 Q28141 Q5R874
TPG DAA21040
AlphaFold O70133 Q5R874 Q28141 Q08211

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks