BMRB Entry 11456

Title:
Solution structure of the N-terminal dsRBD from RNA helicase A
Deposition date:
2011-11-29
Original release date:
2012-08-29
Authors:
Nagata, Takashi; Muto, Yutaka; Tsuda, Kengo; Inoue, Makoto; Kigawa, Takanori; Terada, Takaho; Shirouzu, Mikako; Yokoyama, Shigeyuki
Citation:

Citation: Nagata, Takashi; Tsuda, Kengo; Kobayashi, Naohiro; Shirouzu, Mikako; Kigawa, Takanori; Guntert, Peter; Yokoyama, Shigeyuki; Muto, Yutaka. "Solution structures of the double-stranded RNA-binding domains from RNA helicase A"  Proteins 80, 1699-1706 (2012).
PubMed: 22454253

Assembly members:

Assembly members:
entity, polymer, 99 residues, 10733.203 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: E. coli - cell free   Vector: not applicable

Data sets:
Data typeCount
13C chemical shifts384
15N chemical shifts104
1H chemical shifts622

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA helicase A1

Entities:

Entity 1, RNA helicase A 99 residues - 10733.203 Da.

1   GLYSERSERGLYSERSERGLYILELYSASN
2   PHELEUTYRALATRPCYSGLYLYSARGLYS
3   METTHRPROALATYRGLUILEARGALAVAL
4   GLYASNLYSASNARGGLNLYSPHEMETCYS
5   GLUVALARGVALGLUGLYPHEASNTYRALA
6   GLYMETGLYASNSERTHRASNLYSLYSASP
7   ALAGLNSERASNALAALAARGASPPHEVAL
8   ASNTYRLEUVALARGILEASNGLUVALLYS
9   SERGLUGLUVALPROALAVALGLYILEVAL
10   PROPROPROSERGLYPROSERSERGLY

Samples:

sample_1: entity, [U-100% 13C; U-100% 15N], 1.3 mM; sodium phosphate 20 mM; sodium chloride 100 mM; DTT 1 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm, Guntert, Mumenthaler and Wuthrich - refinement, structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAB28848 BAE24846 BAE41336
GB AAC05301 AAC05725 AAH33911 AAH89159 AAI69284
REF NP_001100654 NP_031868 XP_005363931 XP_005363932 XP_006250057
SP O70133
AlphaFold O70133

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks