BMRB Entry 11375

Title:
Assigned chemical shifts of the second RNA recognition motif (RRM) of TIA-1 with RNA (UUUUU)
Deposition date:
2010-09-08
Original release date:
2010-11-30
Authors:
Takahashi, M.; Kuwasako, K.; Abe, C.; Tsuda, K.; Inoue, M.; Terada, T.; Shirouzu, M.; Kobayashi, N.; Kigawa, T.; Taguchi, S.; Guntert, P.; Hayashizaki, Y.; Tanaka, A.; Muto, Y.; Yokoyama, S.
Citation:

Citation: Kuwasako, Kanako; Takahashi, Mari; Tochio, Naoya; Abe, Chikage; Tsuda, Kengo; Inoue, Makoto; Terada, Takaho; Shirouzu, Mikako; Kobayashi, Naohiro; Kigawa, Takanori; Taguchi, Seiichi; Tanaka, Akiko; Hayashizaki, Yoshihide; Guntert, Peter; Muto, Yutaka; Yokoyama, Shigeyuki. "Solution structure of the second RNA recognition motif (RRM) domain of murine T cell intracellular antigen-1 (TIA-1) and its RNA recognition mode."  Biochemistry 47, 6437-6450 (2008).
PubMed: 18500819

Assembly members:

Assembly members:
RRM domain, polymer, 115 residues, Formula weight is not available
RNA (UUUUU), polymer, 5 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: Escherichia coli

Data sets:
Data typeCount
15N chemical shifts100
1H chemical shifts100

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RRM domain1
2RNA (UUUUU)2

Entities:

Entity 1, RRM domain 115 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLNLYSLYS
2   ASPTHRSERASNHISPHEHISVALPHEVAL
3   GLYASPLEUSERPROGLUILETHRTHRGLU
4   ASPILELYSALAALAPHEALAPROPHEGLY
5   ARGILESERASPALAARGVALVALLYSASP
6   METALATHRGLYLYSSERLYSGLYTYRGLY
7   PHEVALSERPHEPHEASNLYSTRPASPALA
8   GLUASNALAILEGLNGLNMETGLYGLYGLN
9   TRPLEUGLYGLYARGGLNILEARGTHRASN
10   TRPALATHRARGLYSPROPROALAPROLYS
11   SERTHRTYRGLUSERASNTHRLYSGLNSER
12   GLYPROSERSERGLY

Entity 2, RNA (UUUUU) 5 residues - Formula weight is not available

1   UUUUU

Samples:

sample_1: RRM domain, [U-99% 13C; U-99% 15N], 1 mM; RNA (UUUUU) 0.583 mM; TRIS, [U-99% 2H], 20 mM; sodium chloride 100 mM; DTT, [U-98% 2H], 1 mM; sodium azide 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 100 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropiccondition_1
3D 1H-13C NOESYsample_1isotropiccondition_1

Software:

OPAL, Luginbuhl, Guntert, Billeter and Wuthrich - refinement, structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

BMRB 11370 11371 11372 11373 11374 11376
PDB
GB EAW99825
REF XP_005430921

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks