BMRB Entry 11320

Title:
solution structure of the sh3 domain-binding glutamic acid-rich-like protein 2
Deposition date:
2010-08-10
Original release date:
2011-08-19
Authors:
Miyamoto, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Miyamoto, K.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "solution structure of the sh3 domain-binding glutamic acid-rich-like protein 2"  .

Assembly members:

Assembly members:
SH3BGRL2, polymer, 111 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P040614-04

Data sets:
Data typeCount
13C chemical shifts484
15N chemical shifts110
1H chemical shifts755

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SH3BGRL21

Entities:

Entity 1, SH3BGRL2 111 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYMETVALILE
2   ARGVALPHEILEALASERSERSERGLYPHE
3   VALALAILELYSLYSLYSGLNGLNASPVAL
4   VALARGPHELEUGLUALAASNLYSILEGLU
5   PHEGLUGLUVALASPILETHRMETSERGLU
6   GLUGLNARGGLNTRPMETTYRLYSASNVAL
7   PROPROGLULYSLYSPROTHRGLNGLYASN
8   PROLEUPROPROGLNILEPHEASNGLYASP
9   ARGTYRCYSGLYASPTYRASPSERPHEPHE
10   GLUSERLYSGLUSERASNTHRVALPHESER
11   PHELEUGLYLEULYSSERGLYPROSERSER
12   GLY

Samples:

sample_1: SH3BGRL2, [U-13C; U-15N], 0.95 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.925, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 900 MHz

Related Database Links:

PDB
DBJ BAG34700
EMBL CAC35771 CAH89748
GB AAH40489 AAH52987 AAH60799 AAH70059 AAI09044
REF NP_113657 XP_001146260 XP_003258313 XP_003897882 XP_003923315
SP Q5REQ9 Q9UJC5
AlphaFold Q9UJC5 Q5REQ9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks