BMRB Entry 11235

Title:
Solution structure of the 6th Ig-like domain from human Myosin-binding protein C, fast-type
Deposition date:
2010-07-23
Original release date:
2011-08-03
Authors:
Qin, X.; Nagashima, T.; Hayashi, F.; Yokoyama, S.
Citation:

Citation: Qin, X.; Nagashima, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the 6th Ig-like domain from human Myosin-binding protein C, fast-type"  .

Assembly members:

Assembly members:
Ig-like domain, polymer, 118 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P060828-20

Data sets:
Data typeCount
13C chemical shifts515
15N chemical shifts114
1H chemical shifts828

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ig-like domain1

Entities:

Entity 1, Ig-like domain 118 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYTHRLEUALA
2   GLNPROVALTHRILEARGGLUILEALAGLU
3   PROPROLYSILEARGLEUPROARGHISLEU
4   ARGGLNTHRTYRILEARGLYSVALGLYGLU
5   GLNLEUASNLEUVALVALPROPHEGLNGLY
6   LYSPROARGPROGLNVALVALTRPTHRLYS
7   GLYGLYALAPROLEUASPTHRSERARGVAL
8   HISVALARGTHRSERASPPHEASPTHRVAL
9   PHEPHEVALARGGLNALAALAARGSERASP
10   SERGLYGLUTYRGLULEUSERVALGLNILE
11   GLUASNMETLYSASPTHRALATHRILEARG
12   ILEARGVALVALGLULYSALAGLY

Samples:

sample_1: Ig-like domain, [U-13C; U-15N], 1.04 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9816, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks