BMRB Entry 11198

Title:
Solution structure of the third PDZ domain of synapse-associated protein 102
Deposition date:
2010-07-21
Original release date:
2011-07-20
Authors:
Qin, X.; Nagashima, T.; Hayashi, F.; Yokoyama, S.
Citation:

Citation: Qin, X.; Nagashima, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the third PDZ domain of synapse-associated protein 102"  .

Assembly members:

Assembly members:
PDZ domain, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P021030-42

Data sets:
Data typeCount
13C chemical shifts459
15N chemical shifts120
1H chemical shifts728

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1synapse-associated protein 1021

Entities:

Entity 1, synapse-associated protein 102 113 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYARGPROGLY
2   GLYASPALAARGGLUPROARGLYSILEILE
3   LEUHISLYSGLYSERTHRGLYLEUGLYPHE
4   ASNILEVALGLYGLYGLUASPGLYGLUGLY
5   ILEPHEVALSERPHEILELEUALAGLYGLY
6   PROALAASPLEUSERGLYGLULEUARGARG
7   GLYASPARGILELEUSERVALASNGLYVAL
8   ASNLEUARGASNALATHRHISGLUGLNALA
9   ALAALAALALEULYSARGALAGLYGLNSER
10   VALTHRILEVALALAGLNTYRARGPROGLU
11   GLUTYRSERARGPHEGLUSERSERGLYPRO
12   SERSERGLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.05 mM; phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, varian - collection

NMRPipe v20020425, Delaglio., F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.854, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAA86546 BAG64935 BAH14889
GB EAX05333 EAX05335 EDL14166
REF NP_001171250 NP_065781 XP_003484171 XP_003917894 XP_004000645

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks