BMRB Entry 11091

Title:
Solution structure of the C-terminal PapD-like domain from human HYDIN protein
Deposition date:
2010-01-18
Original release date:
2011-01-19
Authors:
Li, H.; Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Li, H.; Sato, M.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the C-terminal PapD-like domain from human HYDIN protein"  .

Assembly members:

Assembly members:
PapD domain, polymer, 112 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: Cell free synthesis   Host organism: E. coli - cell free   Vector: P060327-17

Data sets:
Data typeCount
13C chemical shifts484
15N chemical shifts107
1H chemical shifts756

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PapD domain1

Entities:

Entity 1, PapD domain 112 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYPROLYSILE
2   HISPHEASNPHEGLULEULEUASPILEGLY
3   LYSVALPHETHRGLYSERALAHISCYSTYR
4   GLUALAILELEUTYRASNLYSGLYSERILE
5   ASPALALEUPHEASNMETTHRPROPROTHR
6   SERALALEUGLYALACYSPHEVALPHESER
7   PROLYSGLUGLYILEILEGLUPROSERGLY
8   VALGLNALAILEGLNILESERPHESERSER
9   ILEILELEUGLYASNPHEGLUGLUGLUPHE
10   LEUVALASNVALASNGLYSERPROGLUPRO
11   VALLYSLEUTHRILEARGGLYCYSVALILE
12   GLYPRO

Samples:

sample_1: PapD domain, [U-13C; U-15N], 1.13 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.932, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
DBJ BAB14314 BAG61096 BAG61346
EMBL CAD38687 CAD39007
GB AAH28351 AIC60203 EAW68881
REF NP_001185471 NP_001185472 NP_001257903 NP_060028 XP_004057985
SP Q4G0P3
AlphaFold Q4G0P3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks