BMRB Entry 10324

Name: Solution structure of the 20th Filamin domain from human Filamin-B
Published: 2010-04-07

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PDB ID: 2dlg
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR10324
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the 20th Filamin domain from human Filamin-B

Deposition date:

2009-04-02

Original release date:

2010-04-07

Authors:

Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.

Citation:

Citation: Tomizawa, T.; Koshiba, S.; Watanabe, S.; Harada, T.; Kigawa, T.; Yokoyama, S.. "Solution structure of the 20th Filamin domain from human Filamin-B" .

Assembly members:

Assembly members:
Filamin domain, polymer, 102 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Vector: P051205-17

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Filamin domain: GSSGSSGRAPSVATVGSICD LNLKIPEINSSDMSAHVTSP SGRVTEAEIVPMGKNSHCVR FVPQEMGVHTVSVKYRGQHV TGSPFQFTVGPLGEGGSGPS SG

Data sets:

assigned_chemical_shifts
- chemical_shift_1

Data type	Count
13C chemical shifts	395
15N chemical shifts	89
1H chemical shifts	613

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Filamin domain	1

Entities:

Entity 1, Filamin domain 102 residues - Formula weight is not available

1

GLY

SER

GLY

SER

GLY

ARG

ALA

PRO

2

SER

VAL

ALA

THR

VAL

GLY

SER

ILE

CYS

ASP

3

LEU

ASN

LEU

LYS

ILE

PRO

GLU

ILE

ASN

SER

4

SER

ASP

MET

SER

ALA

HIS

VAL

THR

SER

PRO

5

SER

GLY

ARG

VAL

THR

GLU

ALA

GLU

ILE

VAL

6

PRO

MET

GLY

LYS

ASN

SER

HIS

CYS

VAL

ARG

7

PHE

VAL

PRO

GLN

GLU

MET

GLY

VAL

HIS

THR

8

VAL

SER

VAL

LYS

TYR

ARG

GLY

GLN

HIS

VAL

9

THR

GLY

SER

PRO

PHE

GLN

PHE

THR

VAL

GLY

10

PRO

LEU

GLY

GLU

GLY

SER

GLY

PRO

SER

11

SER

GLY

Samples:

sample_1: Filamin domain, [U-13C; U-15N], 1.14 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	sample_1	isotropic	condition_1
3D 15N-separated NOESY	sample_1	isotropic	condition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9742, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

Bruker AVANCE 800 MHz

PDB	2DLG
GB	AAL68442 AAL68443