BMRB Entry 10091

Title:
Solution structure of the C-terminal ubiquitin-like domain of mouse tubulin-specific chaperone e
Deposition date:
2007-01-24
Original release date:
2008-08-14
Authors:
Sato, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Sato, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the C-terminal ubiquitin-like domain of mouse tubulin-specific chaperone e"  .

Assembly members:

Assembly members:
ubiquitin like-domain, polymer, 97 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P040301-91

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts422
15N chemical shifts96
1H chemical shifts689

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tubulin-folding protein TBCE1

Entities:

Entity 1, Tubulin-folding protein TBCE 97 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLNLEULEU
2   THRLEULYSILELYSCYSSERASNGLNPRO
3   GLUARGGLNILELEUGLULYSGLNLEUPRO
4   ASPSERMETTHRVALGLNLYSVALLYSGLY
5   LEULEUSERARGLEULEULYSVALPROVAL
6   SERGLULEULEULEUSERTYRGLUSERSER
7   LYSMETPROGLYARGGLUILEGLULEUGLU
8   ASNASPLEUGLNPROLEUGLNPHETYRSER
9   VALGLUASNGLYASPCYSLEULEUVALARG
10   TRPSERGLYPROSERSERGLY

Samples:

sample_1: ubiquitin-like domain, [U-13C; U-15N], 1.1 mM; d-Tris-HCl 20 mM; NaCl 200 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 220 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.8994, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 700 MHz

Related Database Links:

PDB
DBJ BAE39475
GB AAH50206 AAL92570 EDL32750 EDL32752 EDL32753
REF NP_848027 XP_006516836 XP_006516838
SP Q8CIV8
AlphaFold Q8CIV8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks