BMRB Entry 10081

Title:
Solution structure of the PDZ domain of mouse Rhophilin-2
Deposition date:
2007-01-23
Original release date:
2008-08-14
Authors:
Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Yoneyama, M.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the PDZ domain of mouse Rhophilin-2"  .

Assembly members:

Assembly members:
PDZ domain, polymer, 111 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P030203-40

Data sets:
Data typeCount
13C chemical shifts427
15N chemical shifts100
1H chemical shifts678

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1rhophilin, Rho GTPase binding protein 21

Entities:

Entity 1, rhophilin, Rho GTPase binding protein 2 111 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYSERALASER
2   LYSARGTRPSERPROPROARGGLYILEHIS
3   PHETHRVALGLUGLUGLYASPLEUGLYPHE
4   THRLEUARGGLYASNTHRPROVALGLNVAL
5   HISPHELEUASPPROHISCYSSERALASER
6   LEUALAGLYALALYSGLUGLYASPTYRILE
7   VALSERILEGLNGLYVALASPCYSLYSTRP
8   LEUTHRVALSERGLUVALMETLYSLEULEU
9   LYSSERPHEGLYGLYGLUGLUVALGLUMET
10   LYSVALVALSERLEULEUASPSERTHRSER
11   SERMETHISASNLYSSERGLYPROSERSER
12   GLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1 mM; acetate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 4.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1not availablecondition_1
3D 13C-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20020425, Delaglio, F. - processing

NMRView v5.0.4, Jhonson, B.A. - data analysis

Kujira v0.891, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks