BMRB Entry 10046

Title:
Solution structure of the PB1 domain of mouse mitogen activated protein kinase kinase 5 (MAP2K5)
Deposition date:
2006-11-09
Original release date:
2008-08-13
Authors:
Yoneyama, M.; Hayashi, F.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Yoneyama, M.; Hayashi, F.; Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the PB1 domain of mouse mitogen activated protein kinase kinase 5 (MAP2K5)"  .

Assembly members:

Assembly members:
PB1 domain, polymer, 113 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P031125-28

Data sets:
Data typeCount
13C chemical shifts473
15N chemical shifts108
1H chemical shifts743

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Mitogen activated protein kinase kinase 51

Entities:

Entity 1, Mitogen activated protein kinase kinase 5 113 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYPROPHECYS
2   ALAMETGLUASNGLNVALLEUVALILEARG
3   ILELYSILEPROASNSERGLYALAVALASP
4   TRPTHRVALHISSERGLYPROGLNLEULEU
5   PHEARGASPVALLEUASPVALILEGLYGLN
6   VALLEUPROGLUALATHRTHRTHRALAPHE
7   GLUTYRGLUASPGLUASPGLYASPARGILE
8   THRVALARGSERASPGLUGLUMETLYSALA
9   METLEUSERTYRTYRTYRSERTHRVALMET
10   GLUGLNGLNVALASNGLYGLNLEUILEGLU
11   PROLEUGLNILEPHEPROARGSERGLYPRO
12   SERSERGLY

Samples:

sample_1: PB1 domain, [U-13C; U-15N], 1 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 297 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1not availablecondition_1
3D 13C-separated NOESYsample_1not availablecondition_1

Software:

VNMR v6.1c, Varian - collection

NMRView v5.0.4, Jhonson, B.A. - data analysis

Kujira v0.896, Kobayashi, N. - data analysis

CYANA v1.0.8, Guntert, P. - refinement, structure solution

NMRPipe v20020425, Delaglio, F. - processing

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAB32187
GB EDL26038 EDL95771 EGW04324 ELK05300 ELW62370

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks