BMRB Entry 10037

Title:
Solution structure of the third mbt domain from human KIAA1798 protein
Deposition date:
2006-11-02
Original release date:
2008-08-13
Authors:
Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.
Citation:

Citation: Tomizawa, T.; Kigawa, T.; Koshiba, S.; Inoue, M.; Yokoyama, S.. "Solution structure of the third mbt domain from human KIAA1798 protein"  .

Assembly members:

Assembly members:
mbt domain, polymer, 107 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P040119-32

Data sets:
Data typeCount
13C chemical shifts439
15N chemical shifts94
1H chemical shifts656

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1KIAA1798 protein1

Entities:

Entity 1, KIAA1798 protein 107 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYVALLYSPRO
2   PROHISGLYPHEGLNLYSLYSMETLYSLEU
3   GLUVALVALASPLYSARGASNPROMETPHE
4   ILEARGVALALATHRVALALAASPTHRASP
5   ASPHISARGVALLYSVALHISPHEASPGLY
6   TRPASNASNCYSTYRASPTYRTRPILEASP
7   ALAASPSERPROASPILEHISPROVALGLY
8   TRPCYSSERLYSTHRGLYHISPROLEUGLN
9   PROPROLEUSERPROLEUGLULEUMETGLU
10   ALASERGLUHISGLYGLYCYSSERTHRPRO
11   GLYSERGLYPROSERSERGLY

Samples:

sample_1: mbt domain, [U-13C; U-15N], 1.18 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.901, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks