BMRB Entry 10029

Title:
Solution structure of the fifth PDZ domain of human membrane associated guanylate kinase inverted-2 (KIAA0705 protein)
Deposition date:
2006-11-01
Original release date:
2008-08-12
Authors:
Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.
Citation:

Citation: Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "Solution structure of the fifth PDZ domain of human membrane associated guanylate kinase inverted-2 (KIAA0705 protein)"  .

Assembly members:

Assembly members:
PDZ domain, polymer, 103 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Vector: P021030-33

Data sets:
Data typeCount
13C chemical shifts417
15N chemical shifts102
1H chemical shifts659

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1membrane associated guanylate kinase inverted-21

Entities:

Entity 1, membrane associated guanylate kinase inverted-2 103 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYGLNASPPHE
2   ASPTYRPHETHRVALASPMETGLULYSGLY
3   ALALYSGLYPHEGLYPHESERILEARGGLY
4   GLYARGGLUTYRLYSMETASPLEUTYRVAL
5   LEUARGLEUALAGLUASPGLYPROALAILE
6   ARGASNGLYARGMETARGVALGLYASPGLN
7   ILEILEGLUILEASNGLYGLUSERTHRARG
8   ASPMETTHRHISALAARGALAILEGLULEU
9   ILELYSSERGLYGLYARGARGVALARGLEU
10   LEULEULYSARGGLYTHRGLYSERGLYPRO
11   SERSERGLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.3 mM; phosphate 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1not availablecondition_1
3D 15N-separated NOESYsample_1not availablecondition_1

Software:

xwinnmr v2.6, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B. A. - data analysis

Kujira v0.8994, Kobayashi, N. - data analysis

CYANA v2.0.17, Guentert, P. - refinement, structure solution

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks